Prevalence rates of Borrelia burgdorferi sensu lato in host-seeking Ixodes ricinus ticks in Europe
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9521004
DOI
10.1007/s004360050378
Knihovny.cz E-resources
- MeSH
- Borrelia isolation & purification MeSH
- Fluorescent Antibody Technique, Indirect MeSH
- Fluorescent Antibody Technique, Direct MeSH
- Ixodes parasitology MeSH
- Microscopy, Phase-Contrast methods MeSH
- Polymerase Chain Reaction MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Europe MeSH
Borrelia burgdorferi sensu lato spirochetes have been found in all examined Ixodes ricinus (L.) populations in Europe. The overall mean proportions of unfed I. ricinus infected with B. burgdorferi s.l. were 1.9% (range 0-11%), 10.8% (2-43%) and 17.4% (3-58%) for larvae (n = 5699), nymphs (n = 48,804) and adults (n = 41,666), respectively. However, the results varied according to the method used. Cultivation in BSK medium is the least sensitive technique (an average of 11% adult ticks found infected), whereas polymerase chain reaction detecting spirochetal DNA is probably the most sensitive method (29% adults found infected). Microscopic methods (dark field, phase contrast, direct or indirect fluorescence) are generally comparable to each other (17-20% adults found infected) and should be regarded as standard procedures because they also make possible a quantitative estimation of spirochetes in the vector. Some technical problems of these methods are discussed.
References provided by Crossref.org
Prevalence of borreliae in ixodid ticks from a floodplain forest ecosystem
