T cell receptor signalling results in rapid tyrosine phosphorylation of the linker protein LAT present in detergent-resistant membrane microdomains
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
9675140
DOI
10.1006/bbrc.1998.8857
PII: S0006291X9898857X
Knihovny.cz E-resources
- MeSH
- Adaptor Proteins, Signal Transducing * MeSH
- Detergents pharmacology MeSH
- Phosphoproteins physiology MeSH
- Phosphorylation MeSH
- Glycosylphosphatidylinositols metabolism MeSH
- Immunoblotting MeSH
- Intracellular Signaling Peptides and Proteins MeSH
- Jurkat Cells MeSH
- Kinetics MeSH
- Humans MeSH
- Membrane Proteins * MeSH
- Proteins analysis MeSH
- Receptors, Antigen, T-Cell physiology MeSH
- Signal Transduction physiology MeSH
- src-Family Kinases physiology MeSH
- Carrier Proteins physiology MeSH
- Tyrosine metabolism MeSH
- Lymphocyte Specific Protein Tyrosine Kinase p56(lck) physiology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Names of Substances
- Adaptor Proteins, Signal Transducing * MeSH
- Detergents MeSH
- Phosphoproteins MeSH
- Glycosylphosphatidylinositols MeSH
- Intracellular Signaling Peptides and Proteins MeSH
- LAT protein, human MeSH Browser
- Membrane Proteins * MeSH
- Proteins MeSH
- Receptors, Antigen, T-Cell MeSH
- SH2B3 protein, human MeSH Browser
- src-Family Kinases MeSH
- Carrier Proteins MeSH
- Tyrosine MeSH
- Lymphocyte Specific Protein Tyrosine Kinase p56(lck) MeSH
Stimulation of human T cell line Jurkat results in rapid tyrosine phosphorylation of a 35-38 kDa protein that is found in large and buoyant detergent-resistant membrane microdomains containing also glycosylphosphatidylinositol (GPI)-anchored proteins, glycolipids and Src-family protein tyrosine kinases ("GPI-microdomains"). The pp35-38 was found to be identical to LAT, a recently cloned key component of the T-cell receptor signalling pathway. Moreover, a modified form of protein tyrosine kinase Lck (pp60) was newly detected in the GPI-microdomains of the anti-CD3-stimulated Jurkat cells. These data support the idea that GPI-microdomains play important roles in immunoreceptor signalling.
References provided by Crossref.org
LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling
