Update on the major clonal types of methicillin-resistant Staphylococcus aureus in the Czech Republic

. 2003 Nov ; 41 (11) : 4998-5005.

Jazyk angličtina Země Spojené státy americké Médium print

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid14605130

The purpose of our study was the molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in 21 hospitals in the Czech Republic in the period 2000-2002 and comparison with previous results from 1996-1997. Strains were analyzed by pulsed-field gel electrophoresis (PFGE) of SmaI digests and ribotyping of HindIII digests hybridized with a 16S-23S DNA probe. The prevalence of the most clinically important macrolide (ermA, ermB, ermC, and msrA) and aminoglycoside (aph3', ant4', and aac6'-aph2") resistance genes was evaluated as well. Selected isolates representative of each clonal type were analyzed by multilocus sequence typing and by a multiplex PCR method capable of identifying the structural type of the staphylococcal cassette chromosome mec (SCCmec) carried by the bacteria. Our results document the displacement of the Brazilian clone (ST239, SCCmec type IIIA, PFGE type B, ribotype H1) by a new clone that we named "Czech clone" (ST239, SCCmec type IIIA, PFGE type F, ribotype H6) and the maintenance of the Iberian clone (ST247, SCCmec type IA, PFGE type A, ribotype H2) exclusively in one hospital in the Czech Republic. In addition, we found a correlation between the distribution of aminoglycoside resistance genes and MRSA clonal types.

Zobrazit více v PubMed

Aires de Sousa, M., M. I. Crisóstomo, I. Santos Sanches, J. S. Wu, J. Fuzhong, A. Tomasz, and H. de Lencastre. 2002. Frequent recovery of a single clonal type of multidrug resistant Staphylococcus aureus from patients in two hospitals in Taiwan and China. J. Clin. Microbiol. 41:159-163. PubMed PMC

Aires de Sousa, M., H. de Lencastre, I. Santos Sanches, K. Kikuchi, K. Totsuka, and A. Tomasz. 2000. Similarity of antibiotic resistance patterns and molecular typing properties of methicillin-resistant Staphylococcus aureus isolates widely spread in hospitals in New York City and in a hospital in Tokyo, Japan. Microb. Drug Resist. 6:253-258. PubMed

Aires de Sousa, M., M. Miragaia, I. Santos Sanches, S. Ávila, I. Adamson, S. T. Casagrande, M. C. C. Brandileone, R. Palacio, L. Dell'Acqua, M. Hortal, T. Camou, A. Rossi, M. E. Velazquez-Meza, G. Echaniz-Aviles, F. Solorzano-Santos, I. Heitmann, and H. de Lencastre. 2001. Three-year assessment of methicillin-resistant Staphylococcus aureus clones in Latin America, 1996-1998. J. Clin. Microbiol. 39:2197-2205. PubMed PMC

Aires de Sousa, M., I. Santos Sanches, M. L. Ferro, M. J. Vaz, Z. Saraiva, T. Tendeiro, J. Serra, and H. de Lencastre. 1998. Intercontinental spread of a multidrug-resistant methicillin-resistant Staphylococcus aureus clone. J. Clin. Microbiol. 36:2590-2596. PubMed PMC

Amorim, M. L., M. Aires de Sousa, I. S. Sanches, R. Sá-Leão, J. M. Cabeda, J. M. Amorim, and H. de Lencastre. 2002. Clonal and antibiotic resistance profiles of methicillin-resistant Staphylococcus aureus (MRSA) from a Portuguese hospital over time. Microb. Drug Resist. 8:301-309. PubMed

Bargs, N. L., and T. Harris. 1997. Toxin-mediated syndromes, p. 527-545. In K. B Crossley and G. L. Archer (ed.), The staphylococci in human disease. Churchill Livingstone, New York, N.Y.

Chung, M., H. de Lencastre, P. Matthews, A. Tomasz, I. Adamsson, M. Aires de Sousa, T. Camou, C. Cocuzza, A. Corso, I. Couto, A. Dominguez, M. Gniadkowski, R. Goering, A. Gomes, K. Kikuchi, A. Marchese, R. Mato, O. Melter, D. Oliveira, R. Palacio, R. Sa-Leao, I. Santos Sanches, J. H. Song, P. T. Tassios, and P. Villari. 2000. Molecular typing of methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis: comparison of results obtained in a multilaboratory effort using identical protocols and MRSA strains. Microb. Drug Resist. 6:189-198. PubMed

Corso, A., I. Santos Sanches, M. Aires de Sousa, A. Rossi, and H. de Lencastre. 1998. Spread of a dominant methicillin-resistant multiresistant Staphylococcus aureus (MRSA) clone in Argentina. Microb. Drug Resist. 4:277-288. PubMed

de Lencastre, H., E. P. Severina, H. Milch, M. Konkoly Thege, and A. Tomasz. 1997. Wide geographic distribution of a unique methicillin-resistant Staphylococcus aureus clone in Hungarian hospitals. Clin. Microbiol. Infect. 3:289-296. PubMed

Dominguez, M. A., H. de Lencastre, J. Liñares, and A. Tomasz. 1994. Spread and maintenance of a dominant methicillin-resistant Staphylococcus aureus (MRSA) clone during an outbreak of MRSA disease in a Spanish hospital. J. Clin. Microbiol. 32:2081-2087. PubMed PMC

Duval, J. 1985. Evolution and epidemiology of MLS resistance. J. Antimicrob. Chemother. 16(Suppl. A):137-149. PubMed

Enright, M. C., N. P. Day, C. E. Davies, S. J. Peacock, and B. G. Spratt. 2000. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J. Clin. Microbiol. 38:1008-1015. PubMed PMC

Enright, M. C., D. A. Robinson, G. Randle, E. J. Feil, H. Grundmann, and B. G. Spratt. 2002. The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA). Proc. Natl. Acad. Sci. USA 99:7687-7692. PubMed PMC

Ida, T., R. Okamoto, C. Shimauchi, T. Okubo, A. Kuga, and M. Inoue. 2001. Identification of aminoglycoside-modifying enzymes by susceptibility testing: epidemiology of methicillin-resistant Staphylococcus aureus in Japan. J. Clin. Microbiol. 39:3115-3121. PubMed PMC

Kreiswirth, B., J. Kornblum, R. D. Arbeit, W. Eisner, J. Maslow, A. McGeer, D. E. Low, and R. Novick. 1993. Evidence for a clonal origin of methicillin resistance in Staphylococcus aureus. Science 259:227-230. PubMed

Leclercq, R., and P. Courvalin. 1991. Bacterial resistance to macrolide, lincosamide, and streptogramin antibiotics by target modification. Antimicrob. Agents Chemother. 35:1267-1272. PubMed PMC

Lina, G., A. Quaglia, M. E. Reverdy, R. Leclercq, F. Vandenesch, and J. Etienne. 1999. Distribution of genes encoding resistance to macrolides, lincosamides, and streptogramins among staphylococci. Antimicrob. Agents Chemother. 43:1062-1066. PubMed PMC

Mato, R., I. Santos Sanches, M. Venditti, D. J. Platt, A. Brown, and H. de Lencastre. 1998. Spread of the multiresistant Iberian clone of methicillin resistance Staphylococcus aureus (MRSA) to Italy and Scotland. Microb. Drug Resist. 4:107-112. PubMed

Melter, O., I. Santos Sanches, J. Schindler, M. Aires de Sousa, R. Mato, V. Kovarova, H. Zemlickova, and H. de Lencastre. 1999. Methicillin-resistant Staphylococcus aureus clonal types in the Czech Republic. J. Clin. Microbiol. 37:2798-2803. PubMed PMC

Moore, P. C. L., and J. A. Lindsay. 2002. Molecular characterization of the dominant UK methicillin-resistant S. aureus strains, EMRSA-15 and EMRSA-16. J. Med. Microbiol. 51:516-521. PubMed

National Committee for Clinical Laboratory Standards. 1995. Performance standards for antimicrobial disk susceptibility test. National Committee for Clinical Laboratory Standards, Wayne, Pa.

National Institute of Public Health and the Environment. 2001. European antimicrobial resistance surveillance system annual report. National Institute of Public Health and the Environment, Bilthoven, The Netherlands.

Oliveira, D. C., I. Crisóstomo, I. Santos Sanches, P. Major, C. R. Alves, M. Aires de Sousa, M. K. Thege, and H. de Lencastre. 2001. Comparison of DNA sequencing of the protein A gene polymorphic region with other molecular typing techniques for typing two epidemiologically diverse collections of methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 39:574-580. PubMed PMC

Oliveira, D. C., and H. de Lencastre. 2002. Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 36:2155-2161. PubMed PMC

Oliveira, D., I. Santos Sanches, R. Mato, M. Tamayo, G. Ribeiro, D. Costa, and H. de Lencastre. 1998. Virtually all methicillin-resistant Staphylococcus aureus (MRSA) infections in the largest Portuguese teaching hospital are caused by two internationally spread multiresistant strains: the “Iberian” and the “Brazilian” clones of MRSA. Clin. Microbiol. Infect. 4:373-384. PubMed

Oliveira, D. C., A. Tomasz, and H. de Lencastre. 2001. The evolution of pandemic clones of methicillin-resistant Staphylococcus aureus: identification of two ancestral genetic backgrounds and the associated mec elements. Microb. Drug Resist. 7:349-361. PubMed

Oliveira, D. C., A. Tomasz, and H. de Lencastre. 2002. Secrets of success of a human pathogen: molecular evolution of pandemic clones of methicillin-resistant Staphylococcus aureus. Lancet Infect. Dis. 2:180-189. PubMed

O′Neill, G. L., S. Murchan, A. Gil-Setas, and H. M. Aucken. 2001. Identification and characterization of phage variants of a strain of epidemic methicillin-resistant S. aureus (EMRSA-15). J. Clin. Microbiol. 39:1540-1548. PubMed PMC

Roberts, R. B., A. M. Tennenberg, W. Eisner, J. Hargrave, L. M. Drusin, R. Yurt, and B. N. Kreiswirth. 1998. Outbreak in a New York city teaching hospital caused by the Iberian epidemic clone of MRSA. Microb. Drug Resist. 4:175-183. PubMed

Ross, J. I., E. A. Eady, J. H. Cove, W. J. Cunliffe, S. Baumberg, and J. C. Wootton. 1990. Inducible erythromycin resistance in staphylococci is encoded by a member of ATP-binding transport super-gene family. Mol. Microbiol. 4:1207-1214. PubMed

Sanches, I. S., M. Ramirez, H. Troni, M. Abecassis, M. Pádua, A. Tomasz, and H. de Lencastre. 1995. Evidence for the geographic spread of a methicillin-resistant Staphylococcus aureus clone between Portugal and Spain. J. Clin. Microbiol. 33:1243-1246. PubMed PMC

Schmitz, F. J., A. C. Fluit, M. Gondolf, R. Beyrau, E. Lindenlauf, J. Verhoef, H. P. Heinz, and M. E. Jones. 1999. The prevalence of aminoglycoside resistance and corresponding resistance genes in clinical isolates of staphylococci from 19 European hospitals. J. Antimicrob. Chemother. 43:253-259. PubMed

Schmitz, F. J., R. Sadurski, A. Kray, M. Boos, R. Geisel, K. Köhrer, J. Verhoef, and A. C. Fluit. 2000. Prevalence of macrolide-resistance genes in Staphylococcus aureus and Enterococcus faecium isolates from 24 European university hospitals. J. Antimicrob. Chemother. 45:891-894. PubMed

Teixeira, L., C. A. Resende, L. R. Ormonde, R. Rosenbaum, A. M. S. Figueiredo, H. de Lencastre, and A. Tomasz. 1995. Geographic spread of epidemic multiresistant Staphylococcus aureus clone in Brazil. J. Clin. Microbiol. 33:2400-2404. PubMed PMC

Tillotson, L. E., W. D. Jenssen, L. Moon-McDermott, and D. T. Dublin. 1989. Characterization of a novel insertion of the macrolide-lincosamide-streptogramin B resistance transposon Tn554 in methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis. Antimicrob. Agents Chemother. 33:541-550. PubMed PMC

Vanhoof, R., C. Godard, J. Content, H. J. Nyssen, and E. Hannecart-Pokorni. 1994. Detection by polymerase chain reaction of genes encoding aminoglycoside-modifying enzymes in methicillin-resistant Staphylococcus aureus isolates of epidemic phage types. J. Med. Microbiol. 41:282-290. PubMed

Westh, H., D. M. Hougaard, J. Vuust, and V. T. Rosdahl. 1995. Prevalence of erm gene classes in erythromycin-resistant Staphylococcus aureus strains isolated between 1959 and 1988. Antimicrob. Agents Chemother. 39:369-373. PubMed PMC

Witte, W., M. Enright, F. J. Schmitz, C. Cuny, C. Braulke, and D. Heuck. 2001. Characteristics of a new epidemic MRSA in Germany ancestral to United Kingdom EMRSA15. Int. J. Med. Microbiol. 290:677-682. PubMed

Nejnovějších 20 citací...

Zobrazit více v
Medvik | PubMed

Prenylated phenolics from Morus alba against MRSA infections as a strategy for wound healing

. 2022 ; 13 () : 1068371. [epub] 20221130

Antibiotic Resistance, spa Typing and Clonal Analysis of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Blood of Patients Hospitalized in the Czech Republic

. 2021 Apr 06 ; 10 (4) : . [epub] 20210406

Epidemiological characteristics of methicillin-resistant Staphylococcus aureus isolates from bloodstream cultures at University Hospital in the Czech Republic

. 2020 Jun ; 65 (3) : 615-622. [epub] 20200314

Methicillin-resistant Staphylococcus aureus in veterinary professionals in 2017 in the Czech Republic

. 2020 Jan 06 ; 16 (1) : 4. [epub] 20200106

Prevalence of resistance mechanisms against macrolides and lincosamides in methicillin-resistant coagulase-negative staphylococci in the Czech Republic and occurrence of an undefined mechanism of resistance to lincosamides

. 2005 Aug ; 49 (8) : 3586-9.

Molecular diagnostics of clinically important staphylococci

. 2004 ; 49 (4) : 353-86.

Najít záznam

Citační ukazatele

Nahrávání dat ...

Možnosti archivace

Nahrávání dat ...