Restriction fragment length polymorphism and virulence of Czech Toxoplasma gondii strains
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9770622
DOI
10.1016/s0020-7519(98)00090-3
PII: S0020-7519(98)00090-3
Knihovny.cz E-resources
- MeSH
- Carnivora MeSH
- Chlorocebus aethiops MeSH
- Fluorescent Antibody Technique, Indirect MeSH
- Genetic Markers MeSH
- Rodentia MeSH
- Cats MeSH
- Rabbits MeSH
- Humans MeSH
- Mice MeSH
- Specific Pathogen-Free Organisms MeSH
- Polymorphism, Restriction Fragment Length * MeSH
- DNA, Protozoan analysis MeSH
- Dogs MeSH
- Birds MeSH
- Cluster Analysis MeSH
- Toxoplasma genetics pathogenicity MeSH
- Vero Cells MeSH
- Virulence MeSH
- Animals MeSH
- Check Tag
- Cats MeSH
- Rabbits MeSH
- Humans MeSH
- Mice MeSH
- Dogs MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- Genetic Markers MeSH
- DNA, Protozoan MeSH
Restriction fragment length polymorphism analysis of chromosomal DNA from 22 strains of Toxoplasma gondii were characterised using SalI and PstI restriction endonucleases and the TGR1E specific repetitive sequence as a probe. Two virulent strains, RH and P-CZ, had previously been isolated from humans, the remaining 20 strains were isolated from animals in the Czech Republic in 1994 and 1995. Among the 20 recently isolated strains, 19 belonged to an avirulent lineage and only one strain from the wild cat Felis silvestris belonged to a virulent lineage.
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