Chromosomal aberrations, sister-chromatid exchanges, cells with high frequency of SCE, micronuclei and comet assay parameters in 1, 3-butadiene-exposed workers
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9804932
DOI
10.1016/s1383-5718(98)00135-1
PII: S1383-5718(98)00135-1
Knihovny.cz E-resources
- MeSH
- Biomarkers MeSH
- Butadienes adverse effects MeSH
- Chromosome Aberrations * MeSH
- Electrophoresis, Agar Gel MeSH
- Glutathione Transferase chemistry MeSH
- Smoking MeSH
- Air Pollutants adverse effects MeSH
- Humans MeSH
- Lymphocytes ultrastructure MeSH
- Micronuclei, Chromosome-Defective MeSH
- Mutagens MeSH
- Polymorphism, Genetic MeSH
- Occupational Exposure MeSH
- Sister Chromatid Exchange MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 1,3-butadiene MeSH Browser
- Biomarkers MeSH
- Butadienes MeSH
- Glutathione Transferase MeSH
- Air Pollutants MeSH
- Mutagens MeSH
The association of occupational exposure to 1,3-butadiene (BD) and induction of cytogenetic damage in peripheral lymphocytes was studied in 19 male workers from a monomer production unit and 19 control subjects from a heat production unit. The exposure to BD was measured by passive personal monitors. The following biomarkers were used: chromosomal aberrations (CA), sister chromatid exchanges (SCE), cells with a high frequency of SCE (HFC), micronuclei, comet assay parameters like tail length (TL) and percentage of DNA in tail [T (%)] and polymorphisms of GSTM1 and GSTT1 genotypes. BD exposure with a median value of 0.53 mg/m3 (range: 0.024-23.0) significantly increased (a) the percentage of cells with chromosomal aberrations in exposed vs. control groups (3.11% vs. 2.03%, P<0.01), (b) the frequency of SCE per cell (6.96 vs. 4.87, P<0.001), and (c) the percentage of HFC (19.9% vs. 4.1%, P<0.001). BD exposure had no significant effects on formation of micronuclei and on comet assay parameters. Effect of smoking was observed only for HFC in BD-exposed group. GSTM1 genotype affected chromosomal aberrations in exposed group, while GSTT1 genotype affected chromosomal aberrations in controls. No effect of GSTM1 or GSTT1 genotypes was observed on any other biomarkers used.
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