A strain of Fusarium oxysporum tolerated copper in the growth medium at concentrations up to 600 mg/L. The optimum growth was obtained at 200 mg Cu/L. The mycelium acquired a blue color in the presence of copper. The copper content of isolated cell walls obtained from mycelium grown in the presence of 600 mg Cu/L was 1.5 times higher than that of cell walls obtained from mycelium grown at 200 mg Cu/L and it contained 2.2 and 3.3% copper at 200 and 600 mg Cu/L, respectively. The amount of protein and total sugars increased in both the mycelium and its isolated cell walls in the presence of copper in the growth medium, chitin was also increased in the cell wall, reaching its maximum amount at 200 mg Cu/L--about 2.4 times higher than without copper. Most of amino acid concentrations in the cell wall were increased in the presence of 200 mg Cu/L and decreased above this concentration. Isoleucine, leucine, tyrosine, phenylalanine, and arginine showed the highest increase at this concentration. The altered cell walls obtained from mycelium grown at 200 and 400 mg Cu/L could rebind individual metals more than the control cell walls could. Rebinding of individual metals was in the order Zn > Fe > Ni > Cu > Co. Rebinding of copper by isolated cell walls depended on pH and temperature.

Botany Department Faculty of Science Menoufia Unviersity Shebin El Kom Egypt

Zobrazit více v PubMed

J Bacteriol. 1975 Jun;122(3):1062-70 PubMed

J Chem Technol Biotechnol. 1990;49(4):345-55 PubMed

J Gen Microbiol. 1981 Jul;125(1):209-12 PubMed

Appl Environ Microbiol. 1983 Jul;46(1):13-6 PubMed

J Chromatogr. 1974 Aug 28;96(1):115-21 PubMed

Appl Microbiol Biotechnol. 1995 Jan;42(5):797-806 PubMed

FEMS Microbiol Rev. 1994 Aug;14(4):325-32 PubMed

Appl Environ Microbiol. 1983 Sep;46(3):585-90 PubMed

Can J Microbiol. 1986 Aug;32(8):654-62 PubMed

Copper-resistant bacteria from industrial effluents and their role in remediation of heavy metals in wastewater

Copper accumulation by Aspergillus awamori