Phospholipase D-neutralization in serodiagnosis of Arcanobacterium haemolyticum and Corynebacterium pseudotuberculosis infections
Jazyk angličtina Země Německo Médium print
Typ dokumentu časopisecké články
- MeSH
- Actinomycetales enzymologie imunologie MeSH
- Corynebacterium pseudotuberculosis enzymologie imunologie MeSH
- fosfolipasa D imunologie metabolismus MeSH
- infekce bakteriemi řádu Actinomycetales diagnóza mikrobiologie MeSH
- korynebakteriální infekce diagnóza mikrobiologie veterinární MeSH
- kozy MeSH
- lidé MeSH
- myši MeSH
- nemoci koz diagnóza mikrobiologie MeSH
- nemoci ovcí diagnóza mikrobiologie MeSH
- neutralizační testy MeSH
- ovce MeSH
- protilátky bakteriální krev MeSH
- sérologické testy MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fosfolipasa D MeSH
- protilátky bakteriální MeSH
Phospholipase D (PLD) neutralization was used to examine sera of humans (n = 40) with a spontaneous infection by Arcanobacterium haemolyticum, sheep and goats (n = 76 and 79 respectively) with a spontaneous infection by Corynebacterium pseudotuberculosis, mice (n = 26) experimentally immunized with PLD from A. haemolyticum (PLD-A) and mice (n = 28) experimentally immunized with PLD from C. pseudotuberculosis (PLD-C). PLD-A and PLD-C were also used as neutralizing antigens. A positive result of neutralization was due to an inhibition of the haemolytic synergism with the equi factor from Rhodococcus equi. The titres of sera neutralizing the homologous PLD were always significantly higher than those neutralizing the heterologous PLD. The proportion of sera that were able to neutralize the homologous PLD in sheep, goats and mice immunized with PLD-A significantly exceeded the proportion of sera that neutralized the heterologous PLD. The antigenic properties of PLD-A and PLD-C were similar but not identical.
Citace poskytuje Crossref.org
