Phospholipase D-neutralization in serodiagnosis of Arcanobacterium haemolyticum and Corynebacterium pseudotuberculosis infections
Language English Country Germany Media print
Document type Journal Article
- MeSH
- Actinomycetales enzymology immunology MeSH
- Corynebacterium pseudotuberculosis enzymology immunology MeSH
- Phospholipase D immunology metabolism MeSH
- Actinomycetales Infections diagnosis microbiology MeSH
- Corynebacterium Infections diagnosis microbiology veterinary MeSH
- Goats MeSH
- Humans MeSH
- Mice MeSH
- Goat Diseases diagnosis microbiology MeSH
- Sheep Diseases diagnosis microbiology MeSH
- Neutralization Tests MeSH
- Sheep MeSH
- Antibodies, Bacterial blood MeSH
- Serologic Tests MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Phospholipase D MeSH
- Antibodies, Bacterial MeSH
Phospholipase D (PLD) neutralization was used to examine sera of humans (n = 40) with a spontaneous infection by Arcanobacterium haemolyticum, sheep and goats (n = 76 and 79 respectively) with a spontaneous infection by Corynebacterium pseudotuberculosis, mice (n = 26) experimentally immunized with PLD from A. haemolyticum (PLD-A) and mice (n = 28) experimentally immunized with PLD from C. pseudotuberculosis (PLD-C). PLD-A and PLD-C were also used as neutralizing antigens. A positive result of neutralization was due to an inhibition of the haemolytic synergism with the equi factor from Rhodococcus equi. The titres of sera neutralizing the homologous PLD were always significantly higher than those neutralizing the heterologous PLD. The proportion of sera that were able to neutralize the homologous PLD in sheep, goats and mice immunized with PLD-A significantly exceeded the proportion of sera that neutralized the heterologous PLD. The antigenic properties of PLD-A and PLD-C were similar but not identical.
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