Inhibition of the rous sarcoma virus long terminal repeat-driven transcription by in vitro methylation: different sensitivity in permissive chicken cells versus mammalian cells
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.
Grant support
1R03-TW00155-01A1
FIC NIH HHS - United States
PubMed
10049832
DOI
10.1006/viro.1998.9597
PII: S0042-6822(98)99597-6
Knihovny.cz E-resources
- MeSH
- Cell Line MeSH
- 3T3 Cells MeSH
- Time Factors MeSH
- DNA, Viral MeSH
- Sarcoma, Experimental MeSH
- Transcription, Genetic MeSH
- Terminal Repeat Sequences * MeSH
- Cricetinae MeSH
- Chickens MeSH
- Chick Embryo MeSH
- DNA Methylation MeSH
- Mice MeSH
- Oncogene Protein pp60(v-src) genetics metabolism MeSH
- Proviruses genetics MeSH
- Gene Expression Regulation, Viral * MeSH
- Transfection MeSH
- Moloney murine leukemia virus genetics MeSH
- Avian Sarcoma Viruses genetics MeSH
- Animals MeSH
- Check Tag
- Cricetinae MeSH
- Chick Embryo MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Names of Substances
- DNA, Viral MeSH
- Oncogene Protein pp60(v-src) MeSH
Rous sarcoma virus (RSV) enhancer sequences in the long terminal repeat (LTR) have previously been shown to be sensitive to CpG methylation. We report further that the high density methylation of the RSV LTR-driven chloramphenicol acetyltransferase reporter is needed for full transcriptional inhibition in chicken embryo fibroblasts and for suppression of tumorigenicity of the RSV proviral DNA in chickens. In nonpermissive mammalian cells, however, the low density methylation is sufficient for full inhibition. The time course of inhibition differs strikingly in avian and mammalian cells: although immediately inhibited in mammalian cells, the methylated RSV LTR-driven reporter is fully inhibited with a significant delay after transfection in avian cells. Moreover, transcriptional inhibition can be overridden by transfection with a high dose of the methylated reporter plasmid in chicken cells but not in hamster cells. The LTR, v-src, LTR proviral DNA is easily capable of inducing sarcomas in chickens but not in hamsters. In contrast, Moloney murine leukemia virus LTR-driven v-src induces sarcomas in hamsters with high incidence. Therefore, the repression of integrated RSV proviruses in rodent cells is directed against the LTR.
References provided by Crossref.org
The Current View of Retroviruses as Seen from the Shoulders of a Giant
Role of DNA methylation in expression and transmission of porcine endogenous retroviruses