We have used the T7 expression system for expression of E. coli FNR protein. The fnr gene was cloned from its initiation codon ATG into the NdeI site of an expression vector and filamentous phage mGP1-2 was used as a donor of T7 RNA polymerase gene. The level of FNR expression attained by this expression arrangement was about 45% of total cell proteins.

Department of Molecular Biology Comenius University Bratislava Slovakia

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Eur J Biochem. 1985 Jan 2;146(1):193-9 PubMed

Mol Microbiol. 1989 May;3(5):601-8 PubMed

Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8 PubMed

Methods Enzymol. 1990;185:60-89 PubMed

J Gen Microbiol. 1987 Dec;133(12):3279-88 PubMed

Antonie Van Leeuwenhoek. 1991 Feb;59(2):65-76 PubMed

Mol Microbiol. 1990 Jan;4(1):21-7 PubMed

Gene. 1987;56(1):125-35 PubMed

FEMS Microbiol Lett. 1992 Feb 1;70(1):31-6 PubMed

Proc Biol Sci. 1991 Sep 23;245(1314):219-26 PubMed

J Biol Chem. 1990 Nov 5;265(31):18733-6 PubMed

Escherichia coli strain with a deletion of the chromosomal ampC gene marked with TcR, suitable for production of penicillin G acylase