Restriction fragment length polymorphism differentiation of plum pox virus isolates
Jazyk angličtina Země Švýcarsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
10073236
Knihovny.cz E-zdroje
- MeSH
- kapsida genetika MeSH
- nemoci rostlin virologie MeSH
- ovoce MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- polymorfismus délky restrikčních fragmentů * MeSH
- RNA virová izolace a purifikace MeSH
- Rosales virologie MeSH
- stromy virologie MeSH
- virus šarky švestky klasifikace genetika izolace a purifikace MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- RNA virová MeSH
Reverse transcription-polymerase chain reaction (RT-PCR) technique and restriction fragment length polymorphism (RFLP) analysis were used to analyse six isolates of plum pox virus (PPV). Whole coat protein (CP) gene was amplified in four isolates using the unipoty-polyT primer pari. PPV-D was identified by RFLP analysis using SfuI and DraI enzymes in two of the isolates. Two isolates of PPV-M strain yielded RT-PCR products which could not be digested by the two enzymes. Other isolates were subjected to RT-PCR using P1-P2 primers. The specificity of the RT-PCR products was confirmed by AluI digestion, while RsaI digestion enabled strain differentiation. No mixed infection was found.
