The analysis of S100A9 and S100A8 expression in matched sets of macroscopically normal colon mucosa and colorectal carcinoma: the S100A9 and S100A8 positive cells underlie and invade tumor mass
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10344284
DOI
10.1002/(sici)1522-2683(19990101)20:4/5<1047::aid-elps1047>3.0.co;2-e
PII: 10.1002/(SICI)1522-2683(19990101)20:4/5<1047::AID-ELPS1047>3.0.CO;2-E
Knihovny.cz E-resources
- MeSH
- Electrophoresis, Gel, Two-Dimensional MeSH
- Antigens, Differentiation, Myelomonocytic analysis MeSH
- Antigens, CD analysis MeSH
- Antigens, Differentiation analysis MeSH
- Immunoenzyme Techniques MeSH
- Neoplasm Invasiveness MeSH
- Calgranulin A MeSH
- Calgranulin B MeSH
- Colon chemistry pathology MeSH
- Colorectal Neoplasms chemistry pathology MeSH
- Humans MeSH
- S100 Proteins analysis MeSH
- Calcium-Binding Proteins analysis MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
- Intestinal Mucosa chemistry pathology MeSH
- Case-Control Studies MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, Differentiation, Myelomonocytic MeSH
- Antigens, CD MeSH
- CD68 antigen, human MeSH Browser
- Antigens, Differentiation MeSH
- Calgranulin A MeSH
- Calgranulin B MeSH
- S100 Proteins MeSH
- Calcium-Binding Proteins MeSH
The expression of calcium-binding protein S100A9 was investigated in 23 matched sets of colorectal carcinoma and normal colon mucosa using two-dimensional gel electrophoresis. We found that, from a group of 23 patients, the level of S100A9 protein, in comparison with matched normal colon mucosa, was significantly increased in malignant tissues of 16 patients (70%). Furthermore, an additional protein, identified by matrix-assisted laser desorption/ionization - mass spectrometry (MALDI-MS) as S100A8, exhibited an increased expression in the same specimens of malignant tissues as the S100A9 protein. The immunohistological analysis revealed the accumulation of S100A9 positive cells, macrophages and polymorphonuclear leukocytes along the invasive margin of colorectal carcinoma. The S100A8 protein was found to be produced in the same location. The possible participation of both proteins and, especially, its heterodimeric complex calprotectin in colorectal carcinoma regression could be taken into account.
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