Identification and determination of succinyladenosine in human cerebrospinal fluid
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Adenosine analogs & derivatives cerebrospinal fluid MeSH
- Chromatography, Thin Layer MeSH
- Child MeSH
- Mass Spectrometry MeSH
- Humans MeSH
- Purine-Pyrimidine Metabolism, Inborn Errors cerebrospinal fluid MeSH
- Reference Standards MeSH
- Reproducibility of Results MeSH
- Spectrophotometry, Ultraviolet MeSH
- Chromatography, High Pressure Liquid MeSH
- Check Tag
- Child MeSH
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adenosine MeSH
- succinyladenosine MeSH Browser
Succinyladenosine (S-Ado) is a biochemical marker of adenylosuccinase deficiency--the genetic defect of purine de novo synthesis. S-Ado has been previously reported as normally undetectable in cerebrospinal fluid (CSF) of children not suffering from this defect. In present study, we employed solid-phase extraction and thin-layer chromatography for isolation of a compound with spectral and chromatographic characteristics identical to S-Ado from human CSF. The high-performance liquid chromatography-negative-ion electrospray ionization mass spectrometry analysis confirmed that the isolated compound is S-Ado. We established the reference values of S-Ado in CSF of children (1.1+/-0.4 micromol/l; mean +/- S.D; n = 26) by means of reversed-phase HPLC method on a C18 column with UV detection.
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