A putative host cell receptor for tick-borne encephalitis virus identified by anti-idiotypic antibodies and virus affinoblotting
Language English Country Switzerland Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10393498
DOI
10.1159/000024954
PII: 24954
Knihovny.cz E-resources
- MeSH
- Antigens, Viral immunology metabolism MeSH
- Cell Line metabolism MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Immunoblotting MeSH
- Cells, Cultured MeSH
- Antibodies, Monoclonal immunology metabolism MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Specific Pathogen-Free Organisms MeSH
- Antibodies, Anti-Idiotypic immunology metabolism MeSH
- Receptors, Virus chemistry MeSH
- Encephalitis Viruses, Tick-Borne chemistry immunology pathogenicity MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, Viral MeSH
- Antibodies, Monoclonal MeSH
- Antibodies, Anti-Idiotypic MeSH
- Receptors, Virus MeSH
Anti-idotypic monoclonal antibodies (anti-ID MAbs) were made against two mouse MAbs that neutralize the infectivity of the tick-borne encephalitis (TBE) virus. Three of the anti-ID MAbs (1) inhibited the binding of respective idiotypic MAb to the TBE virus antigen, (2) inhibited the infectivity of TBE virus when preincubated with virus-susceptible cells, and (3) bound to the surface of virus-susceptible but not virus-nonsusceptible cells. They recognized a 35-kD protein in immunoblotting analysis. Identification of this protein as a component of a putative TBE virus receptor was supported by the viroblot technique. In this assay, two polypeptide signals of 35 and 18 kD were obtained after incubation of blotted cell membrane proteins with the TBE virion antigen.
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History of Arbovirus Research in the Czech Republic
