Overexpression of the G protein G11alpha prevents desensitization of Ca2+ response to thyrotropin-releasing hormone
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
10465349
DOI
10.1016/s0024-3205(99)00319-7
PII: S0024320599003197
Knihovny.cz E-zdroje
- MeSH
- blokátory kalciových kanálů farmakologie MeSH
- bradykinin farmakologie MeSH
- buněčné linie MeSH
- časové faktory MeSH
- EGTA farmakologie MeSH
- exprese genu MeSH
- hormon uvolňující thyreotropin agonisté antagonisté a inhibitory farmakologie MeSH
- inositolfosfáty metabolismus MeSH
- krysa rodu Rattus MeSH
- lidé MeSH
- nifedipin farmakologie MeSH
- proteiny vázající GTP antagonisté a inhibitory genetika metabolismus MeSH
- receptory thyroliberinu agonisté antagonisté a inhibitory genetika fyziologie MeSH
- suramin farmakologie MeSH
- thapsigargin farmakologie MeSH
- transfekce MeSH
- vápník metabolismus farmakologie MeSH
- vápníková signalizace účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- blokátory kalciových kanálů MeSH
- bradykinin MeSH
- EGTA MeSH
- hormon uvolňující thyreotropin MeSH
- inositolfosfáty MeSH
- nifedipin MeSH
- proteiny vázající GTP MeSH
- receptory thyroliberinu MeSH
- suramin MeSH
- thapsigargin MeSH
- vápník MeSH
Doubly transfected human embryonal kidney cells (clone E2M11 of the HEK 293 cell line) expressing both thyrotropin-releasing hormone (TRH) receptors and G11alpha protein in high amounts were used to analyze the desensitization phenomenon of the Ca2+-mobilizing pathway. Quite unexpectedly, we did not observe any significant desensitization of the [Ca2+]i response to TRH in these cells after repeated or prolonged incubation with the hormone (up to 5 h). Under the same conditions, the TRH-induced [Ca2+]i response was completely desensitized in the parent cell line (293-E2 cels) expressing TRH receptors alone. In both cell lines, inositol phosphate response was desensitized after TRH exposure, although basal levels of inositol phospates in TRH-pretreated cells were much higher than in "naive" TRH-unexposed cells. These data suggest a significant role of the G protein G11alpha in desensitization of the Ca2+-mobilizing pathway occuring after repeated or long-term exposure of target cells to TRH-receptor agonists.
Citace poskytuje Crossref.org
Biochemical and physiological insights into TRH receptor-mediated signaling
