Granulocyte colony-stimulating factor and drugs elevating extracellular adenosine act additively to enhance the hemopoietic spleen colony formation in irradiated mice
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10470864
Knihovny.cz E-resources
- MeSH
- Adenosine metabolism MeSH
- Adenosine Monophosphate pharmacology MeSH
- Colony-Forming Units Assay MeSH
- Whole-Body Irradiation * MeSH
- Dipyridamole pharmacology MeSH
- Erythroid Precursor Cells cytology MeSH
- Extracellular Space metabolism MeSH
- Granulocyte Colony-Stimulating Factor pharmacology MeSH
- Hematopoietic Stem Cells cytology MeSH
- Hematopoiesis drug effects MeSH
- Mice MeSH
- Cell Count MeSH
- Cobalt Radioisotopes MeSH
- Spleen cytology MeSH
- Gamma Rays MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adenosine MeSH
- Adenosine Monophosphate MeSH
- Dipyridamole MeSH
- Granulocyte Colony-Stimulating Factor MeSH
- Cobalt Radioisotopes MeSH
The effects of combined administration of two drugs elevating extracellular adenosine, namely dipyridamole (DP) and adenosine monophosphate (AMP), and granulocyte colony-stimulating factor (G-CSF) on hemopoietic stem cells in vivo were investigated. The experiments were performed on mice using the endogenous spleen colony formation in gamma-irradiated animals as an endpoint. The results have shown that DP and AMP act additively with G-CSF to enhance spleen colony formation and thus the erythroid repopulation of the spleen. These findings indicate that the signaling pathways of G-CSF and drugs elevating extracellular adenosine can interact at the level of primitive hemopoietic stem cells. The enhancement of hemopoiesis-stimulating effects of G-CSF by DP and AMP, which are low-priced and clinically available drugs, could improve the cost-effectiveness of the therapy with G-CSF.
Granulocyte colony-stimulating factor in the treatment of acute radiation syndrome: a concise review
The role of adenosine receptor agonists in regulation of hematopoiesis
