Molecular cloning and expression of the human and mouse homologues of the Drosophila dachshund gene
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10502110
DOI
10.1007/s004270050286
PII: 92090537.427
Knihovny.cz E-resources
- MeSH
- Adult MeSH
- Drosophila genetics MeSH
- Embryonic and Fetal Development * MeSH
- Transcription, Genetic * MeSH
- Nuclear Proteins biosynthesis chemistry genetics MeSH
- Cloning, Molecular MeSH
- Humans MeSH
- RNA, Messenger genetics MeSH
- Molecular Sequence Data MeSH
- Mice MeSH
- Organ Specificity MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Drosophila Proteins * MeSH
- Gene Expression Regulation * MeSH
- Recombinant Proteins biosynthesis chemistry MeSH
- Amino Acid Sequence MeSH
- Sequence Homology, Amino Acid MeSH
- Sequence Alignment MeSH
- Pregnancy MeSH
- Gene Expression Regulation, Developmental MeSH
- Animals MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Mice MeSH
- Pregnancy MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- dac protein, Drosophila MeSH Browser
- Nuclear Proteins MeSH
- RNA, Messenger MeSH
- Drosophila Proteins * MeSH
- Recombinant Proteins MeSH
Recent genetic analysis of the Drosophila dachshund (dac) gene has established that dac encodes a novel nuclear protein that is involved in both eye and leg development. In the Drosophila eye, dac expression appears to be controlled by the product of the eyeless/Pax6 gene. In order to analyze the Pax6 pathway in vertebrates we have isolated and characterized the cDNA and genomic clones corresponding to the human and mouse homologues of Drosophila dac. A full-length human cDNA encoding dachshund (DACH) encodes the 706 amino acids protein with predicted molecular weight of 73 kDa. A 109 amino acid domain located at the N-terminus of the DACH showed significant sequence and secondary structure homologies to the ski/sno oncogene products. Northern blot analysis found human DACH predominantly in adult kidney, heart, and placenta, with less expression detected in the brain, lung, skeletal muscle and pancreas. A panel of human cell lines was studied and most notably a large proportion of neuroblastomas expressed DACH mRNA. Mouse Dach encodes a protein of 751 amino acids with predicted molecular weight of 78 kDa that is 95% identical to the human DACH. RNase protection analysis showed the highest Dach mRNA expression in the adult mouse kidney and lung, whereas lower expression was detected in the brain and testis. RT/PCR analysis readily detected Dach mRNA in the adult mouse cornea and retina. Dach mRNA expression in the mouse E11.5 embryo was observed primarily in the fore and hind limbs, as well as in the somites.
References provided by Crossref.org
GENBANK
AF102546, AF102547
