Application of Pluronic copolymer liquid crystals for the capillary electrophoretic separation of collagen type I cyanogen bromide fragments
Language English Country Netherlands Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Cyanogen Bromide chemistry isolation & purification MeSH
- Electrophoresis, Capillary methods MeSH
- Collagen isolation & purification MeSH
- Rats MeSH
- Peptide Fragments isolation & purification MeSH
- Polyethylenes chemistry MeSH
- Polypropylenes chemistry MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Cyanogen Bromide MeSH
- Collagen MeSH
- Peptide Fragments MeSH
- Polyethylenes MeSH
- Polypropylenes MeSH
- UCON 50-HB-5100 MeSH Browser
A capillary electrophoretic method exploiting the properties of Pluronic copolymer liquid crystals (F127) was developed for the separation of collagen cyanogen bromide (CNBr) fragments. The separations obtained were at least comparable (if not better) to those obtained by other methods applicable to this category of compounds. In the optimized version a bare silica capillary [47 cm (40 cm to the detector) x 75 microm I.D.] was used with 10 mM Tris and 75 mM phosphate buffer (pH 2.5) containing 7.5% Pluronic F127 copolymer. The separation mechanism which involves both the molecular sieving and surfactant properties of the Pluronic F127 gel phase is discussed.
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