Determination of isoprene in human expired breath using solid-phase microextraction and gas chromatography-mass spectrometry
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- butadieny analýza MeSH
- časové faktory MeSH
- dechové testy metody MeSH
- hemiterpeny * MeSH
- kalibrace MeSH
- lidé MeSH
- oxid uhličitý chemie MeSH
- pentany * MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí metody MeSH
- reprodukovatelnost výsledků MeSH
- řízení kvality MeSH
- stabilita léku MeSH
- teplota MeSH
- vlhkost MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- butadieny MeSH
- hemiterpeny * MeSH
- isoprene MeSH Prohlížeč
- oxid uhličitý MeSH
- pentany * MeSH
An analytical method for determination of isoprene in expired breath as a marker of body cholesterol synthesis was developed with a special emphasis on breath sampling. Patients were breathing controlled air using respiratory masks for 2 min (washout period) and then their expired breath was collected in 8-1 Tedlar bags. The bags were heated to 40 degrees C and the solid-phase microextraction fiber Carboxen-polydimethylsiloxane 75 microm was inserted through the septum. Extraction time was 10 min. Analytes were desorbed in the GC injector for 2 min at 270 degrees C. Analyses were performed on a Q-PLOT column and fragment ions 68, 67 and 53 were quantified. The concentration range was 1-40 nmol/l, limit of detection was 0.25 nmol/l, the calibration curve was linear. Precision, expressed as RSD, was 5.5-12.5%. These tests are non-invasive, feasible and relatively inexpensive.
Citace poskytuje Crossref.org
