Activation of pig oocytes using calcium ionophore: effect of protein synthesis inhibitor cycloheximide
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
10967244
DOI
10.1016/s0378-4320(00)00150-0
PII: S0378-4320(00)00150-0
Knihovny.cz E-zdroje
- MeSH
- calcimycin farmakologie MeSH
- cykloheximid farmakologie MeSH
- inhibitory syntézy proteinů farmakologie MeSH
- ionofory farmakologie MeSH
- kultivované buňky MeSH
- oocyty účinky léků fyziologie MeSH
- partenogeneze MeSH
- prasata * MeSH
- stadium rýhování vajíčka účinky léků MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- calcimycin MeSH
- cykloheximid MeSH
- inhibitory syntézy proteinů MeSH
- ionofory MeSH
In vitro matured pig oocytes were activated using a combined treatment of calcium ionophore A 23187 with cycloheximide. The oocytes were exposed to ionophore (10, 25 or 50 microM) for 0.5, 1, 3, 5 or 7 min and then cultured with cycloheximide (0 or 10 microg/ml) for 6 h. Cycloheximide treatment significantly increased the activation rate of oocytes and the percentage of oocytes that were able to develop after activation. The highest activation rate was observed after treatment with 50 microM ionophore. The highest percentage of developing eggs was observed after combined treatment of ionophore (25 microM) with cycloheximide. The percentage of oocytes developing up to the morula and blastocyst stage was not significantly increased after cycloheximide treatment.
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