Activation of pig oocytes using calcium ionophore: effect of protein synthesis inhibitor cycloheximide
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10967244
DOI
10.1016/s0378-4320(00)00150-0
PII: S0378-4320(00)00150-0
Knihovny.cz E-resources
- MeSH
- Calcimycin pharmacology MeSH
- Cycloheximide pharmacology MeSH
- Protein Synthesis Inhibitors pharmacology MeSH
- Ionophores pharmacology MeSH
- Cells, Cultured MeSH
- Oocytes drug effects physiology MeSH
- Parthenogenesis MeSH
- Swine * MeSH
- Cleavage Stage, Ovum drug effects MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Calcimycin MeSH
- Cycloheximide MeSH
- Protein Synthesis Inhibitors MeSH
- Ionophores MeSH
In vitro matured pig oocytes were activated using a combined treatment of calcium ionophore A 23187 with cycloheximide. The oocytes were exposed to ionophore (10, 25 or 50 microM) for 0.5, 1, 3, 5 or 7 min and then cultured with cycloheximide (0 or 10 microg/ml) for 6 h. Cycloheximide treatment significantly increased the activation rate of oocytes and the percentage of oocytes that were able to develop after activation. The highest activation rate was observed after treatment with 50 microM ionophore. The highest percentage of developing eggs was observed after combined treatment of ionophore (25 microM) with cycloheximide. The percentage of oocytes developing up to the morula and blastocyst stage was not significantly increased after cycloheximide treatment.
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