The supply of exogenous deoxyribonucleotides accelerates the speed of the replication fork in early S-phase
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11171380
DOI
10.1242/jcs.114.4.747
Knihovny.cz E-resources
- MeSH
- Deoxyribonucleotides pharmacology MeSH
- Microscopy, Fluorescence MeSH
- HeLa Cells MeSH
- Kinetics MeSH
- Humans MeSH
- DNA Replication drug effects MeSH
- S Phase drug effects MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Deoxyribonucleotides MeSH
Earlier studies have established that the average speed of a replication fork is two to three times slower in early S-phase than in late S-phase and that the intracellular 2'-deoxyribonucleoside 5'-triphosphate pools grow during S-phase. In this study, the effect of the exogenous 2'-deoxyribonucleoside 5'-triphosphate (dNTP) supply on the average replication speed in a synchronised population of human HeLa cells was tested. The speed of replication fork movement was measured on extended DNA fibers labelled with 2'-deoxythymidine analogues 5-chloro-2'-deoxyuridine and 5-iodo-2'-deoxyuridine. We show that the introduction of exogenous dNTPs accelerates the replication process at the beginning of DNA synthesis only. In late S-phase, the administration of additional dNTPs has no effect on the speed of replication forks. The availability of 2'-deoxynucleotides seems to be a rate-limiting factor for DNA replication during early S-phase.
References provided by Crossref.org
DNA Replication: From Radioisotopes to Click Chemistry
Organization of human replicon: singles or zipping couples?
