The supply of exogenous deoxyribonucleotides accelerates the speed of the replication fork in early S-phase
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
11171380
DOI
10.1242/jcs.114.4.747
Knihovny.cz E-zdroje
- MeSH
- deoxyribonukleotidy farmakologie MeSH
- fluorescenční mikroskopie MeSH
- HeLa buňky MeSH
- kinetika MeSH
- lidé MeSH
- replikace DNA účinky léků MeSH
- S fáze účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- deoxyribonukleotidy MeSH
Earlier studies have established that the average speed of a replication fork is two to three times slower in early S-phase than in late S-phase and that the intracellular 2'-deoxyribonucleoside 5'-triphosphate pools grow during S-phase. In this study, the effect of the exogenous 2'-deoxyribonucleoside 5'-triphosphate (dNTP) supply on the average replication speed in a synchronised population of human HeLa cells was tested. The speed of replication fork movement was measured on extended DNA fibers labelled with 2'-deoxythymidine analogues 5-chloro-2'-deoxyuridine and 5-iodo-2'-deoxyuridine. We show that the introduction of exogenous dNTPs accelerates the replication process at the beginning of DNA synthesis only. In late S-phase, the administration of additional dNTPs has no effect on the speed of replication forks. The availability of 2'-deoxynucleotides seems to be a rate-limiting factor for DNA replication during early S-phase.
Citace poskytuje Crossref.org
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