Effect of the carbon source on assessment of degrading bacteria with the spread-plating technique during in situ bioremediation
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11200669
DOI
10.1007/bf02817447
Knihovny.cz E-resources
- MeSH
- Bacteria isolation & purification metabolism MeSH
- Bacteriological Techniques * MeSH
- Biodegradation, Environmental MeSH
- Culture Media MeSH
- Environmental Microbiology * MeSH
- Oils metabolism MeSH
- Carbon MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Culture Media MeSH
- Oils MeSH
- Carbon MeSH
Spread-plating belongs to traditional microbiological methods employed for quantification of subsurface microflora during bioremediation projects in the Czechia. Concentration of degrading organisms is estimated from the number of colonies grown on agar plates supplied with contaminant as the sole carbon source. The data obtained during in situ bioremediation of the Dacice site contaminated by cutting oil suggests that changes in the composition of the carbon source in the subsurface may cause a discrepancy between laboratory data and situation in subsurface.
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