Influence of citrate and EDTA anticoagulants on plasma malondialdehyde concentrations estimated by high-performance liquid chromatography
Language English Country Netherlands Media print
Document type Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Anticoagulants pharmacology MeSH
- Adult MeSH
- Edetic Acid pharmacology MeSH
- Calibration MeSH
- Citric Acid pharmacology MeSH
- Middle Aged MeSH
- Humans MeSH
- Malondialdehyde blood MeSH
- Blood Specimen Collection MeSH
- Oxidative Stress MeSH
- Thiobarbiturates blood MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Clinical Trial MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anticoagulants MeSH
- Edetic Acid MeSH
- Citric Acid MeSH
- Malondialdehyde MeSH
- Thiobarbiturates MeSH
- thiobarbituric acid MeSH Browser
Estimation of lipid peroxidation through MDA formation measured by assaying thiobarbituric acid (TBA) reactive products separated by HPLC remains the method of choice to study the development of oxidative stress in blood plasma. In this report we describe the influence of citrate and EDTA anticoagulants used for blood collection on estimation of MDA concentrations using HPLC analysis of MDA-TBA adducts. We analyzed a group of 40 blood donors (21 men and 19 women), median age 27 years, range 19-48 years. The mean MDA concentration in citrate plasma was 1.43+/-0.51 micromol/l (range: 0.61-2.57 micromol/l) and in EDTA plasma 0.36+/-0.10 micromol/l (range: 0.13-0.63 micromol/l). There was a significant difference in MDA mean concentration that we attribute to different antioxidant properties of anticoagulants used for blood collection. Consistency in the choice of anticoagulant is clearly extremely important.
References provided by Crossref.org
Antioxidants change platelet responses to various stimulating events
