Progesterone side-chain degradation by some species of Aspergillus flavus group
Language English Country United States Media print
Document type Journal Article
PubMed
11271808
DOI
10.1007/bf02908952
Knihovny.cz E-resources
- MeSH
- Androstenes metabolism MeSH
- Aspergillus flavus classification growth & development metabolism MeSH
- Mycological Typing Techniques MeSH
- Progesterone chemistry metabolism MeSH
- Spectrophotometry, Infrared MeSH
- Testolactone metabolism MeSH
- Testosterone metabolism MeSH
- Chromatography, High Pressure Liquid MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Androstenes MeSH
- Progesterone MeSH
- Testolactone MeSH
- Testosterone MeSH
Seventy isolates belonging to 6 species and one variety of A. flavus group were shown to degrade the progesterone side-chain to yield delta 4-androstene-3,17-dione and testosterone. The isolates of five species (A. flavo-furcatis, A. flavus, A. oryzae, A. parasiticus and A. tamarii) possessed enzyme systems catalyzing the opening of ring D and formed testololactone as final steroid metabolite in addition to their ability to produce the above mentioned two products. 11 beta-Hydroxy-delta 4-androstene-3,17-dione was formed by only A. flavus and A. tamarii while 11 beta-hydroxytestosterone was produced by A. flavo-furcatis, A. parasiticus and A. subolivaceus. The chromatographic resolution of the mixture products obtained (when the selective isolate of each species reacted with 1 g of progesterone) revealed that 60-75% of progesterone was converted into delta 4-androstene-3,17-dione (8-30%), testosterone (7-33%), testololactone (14-37%) and other products (3-40%). The most bioconversion activity was exhibited by A. oryzae, followed by A. parasiticus. The highest values of delta 4-androstene-3,17-dione (30% of added progesterone) and testosterone (33%) were formed by A. flavus var. columnaris while those of testololactone (37%) were produced by A. oryzae. A systematic variation could be observed between the different tested species of A. flavus group with respect to the transformation reactions of progesterone. Comparative biotransformation results showed that essential differences exist between the tested species in this group; this biochemical differentiation may supplement the morphological and other physiological criteria used in the identification of the different species in the A. flavus group.
See more in PubMed
J Org Chem. 1965 Mar;30:760-2 PubMed
Folia Microbiol (Praha). 1962 Mar;7:121-5 PubMed
J Bacteriol. 1957 Nov;74(5):684-8 PubMed
Steroids. 1997 Apr;62(4):332-45 PubMed
Folia Microbiol (Praha). 1994;39(5):415-9 PubMed
J Basic Microbiol. 1985;25(7):461-74 PubMed
Cesk Farm. 1955 Mar;4(2):65-8 PubMed
Lett Appl Microbiol. 1995 Apr;20(4):243-6 PubMed
Folia Microbiol (Praha). 1974;19(5):378-80 PubMed
Hydroxylation of progesterone by some Trichoderma species
