Dimethylsulfoxide-stabilized conformer of guanine-adenine repeat strand of DNA
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11288056
DOI
10.1002/bip.1000
Knihovny.cz E-resources
- MeSH
- Adenine MeSH
- Circular Dichroism MeSH
- Dimethyl Sulfoxide pharmacology MeSH
- Guanine MeSH
- DNA, Single-Stranded chemistry MeSH
- Nucleic Acid Conformation drug effects MeSH
- Repetitive Sequences, Nucleic Acid * MeSH
- Drug Stability MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adenine MeSH
- Dimethyl Sulfoxide MeSH
- Guanine MeSH
- DNA, Single-Stranded MeSH
Circular dichroism spectroscopy and other methods are used to show that the addition of dimethylsulfoxide causes reversible folding of the (GA)(10) strand of DNA into an ordered single-stranded conformer. The ordered conformer melts in a cooperative way and it does not contain protonated adenine. The (TA)(10), (A)(20), and (G)(20) are all unstable in this conformer. To the best of our knowledge, this is the first known ordered conformer of DNA that is stabilized by dimethylsulfoxide. This conformer might be a DNA analog of the protein alpha helix, which is an interesting idea for thinking about the evolution of DNA.
References provided by Crossref.org
Circular dichroism and conformational polymorphism of DNA
DNA homoduplexes containing no pyrimidine nucleotide
