Detection of antibodies to ovine lentivirus using recombinant capsid and transmembrane proteins
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Antigens, Viral immunology MeSH
- DNA Primers MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Capsid immunology MeSH
- Membrane Proteins immunology MeSH
- Sheep MeSH
- Polymerase Chain Reaction veterinary MeSH
- Predictive Value of Tests MeSH
- Pneumonia, Progressive Interstitial, of Sheep diagnosis MeSH
- Viral Matrix Proteins immunology MeSH
- Antibodies, Viral blood immunology MeSH
- Recombinant Proteins immunology MeSH
- Sensitivity and Specificity MeSH
- Visna-maedi virus immunology isolation & purification MeSH
- Blotting, Western MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, Viral MeSH
- DNA Primers MeSH
- Membrane Proteins MeSH
- Viral Matrix Proteins MeSH
- Antibodies, Viral MeSH
- Recombinant Proteins MeSH
The coding sequences of the capsid protein p25 and transmembrane protein of Maedi-Visna virus were amplified using polymerase chain reaction and cloned into the plasmid expression vector pRSET-B. Both DNA constructs expressed proteins tagged with polyhistidine. The recombinant proteins were purified using Ni-NTA agarose and used in immunoblot to detect antibodies against Maedi-Visna virus. A total of 260 ovine serum specimens was analysed. The total number of p25-positive sera was 111 (42.7%). Higher sensitivity was achieved with rTM antigen, which detected antibodies in 118 (45.4%) sera. The combination of both recombinant proteins as antigens resulted in higher sensitivity of serological detection compared to whole virus antigen.
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