Modulatory effect of cyclosporin A on tert-butyl hydroperoxide-induced oxidative damage in hepatocytes
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11322648
DOI
10.1081/iph-100102566
Knihovny.cz E-resources
- MeSH
- Alanine Transaminase metabolism MeSH
- Cyclosporine pharmacology MeSH
- Cytosol drug effects MeSH
- Hepatocytes drug effects MeSH
- Rats MeSH
- Membrane Potentials drug effects MeSH
- Mitochondria drug effects MeSH
- Oxidative Stress drug effects MeSH
- Rats, Wistar MeSH
- In Vitro Techniques MeSH
- tert-Butylhydroperoxide pharmacology MeSH
- Calcium physiology MeSH
- Cell Survival drug effects MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Alanine Transaminase MeSH
- Cyclosporine MeSH
- tert-Butylhydroperoxide MeSH
- Calcium MeSH
In the present work, we followed an in vitro protective action of cyclosporin A (CsA) against tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in hepatocytes. Various parameters (cell viability, cytosolic calcium level, rhodamine 123 accumulation as indicator of mitochondrial membrane potential and alanine-aminotransferase leakage from cells) were measured as an index of cytotoxicity. Tert-butyl hydroperoxide (1 mM) significantly increased cytosolic Ca2+ and affected mitochondrial membrane potential. Pretreatment with cyclosporin A (0.5 microM) reduced t-BHP-induced cytosolic Ca2+ increase and ALT (alanine-aminotransferase) leakage, but had no protective effect on t-BHP-induced changes of mitochondrial membrane potential. Our data thus suggest that the mechanism of cytoprotection of CsA on the cytosolic Ca2+ changes and ALT leakage induced by t-BHP, does not directly correlate with protection of t-BHP-induced changes of mitochondrial membrane potential.
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