Duration of non-Insulin-dependent diabetes mellitus and the TNF-beta NcoI genotype as predictive factors in proliferative diabetic retinopathy
Language English Country Switzerland Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11399938
DOI
10.1159/000050877
PII: 50877
Knihovny.cz E-resources
- MeSH
- Alleles MeSH
- Peptidyl-Dipeptidase A genetics MeSH
- Time Factors MeSH
- Diabetes Mellitus, Type 2 genetics MeSH
- Diabetic Retinopathy genetics MeSH
- DNA Primers chemistry MeSH
- Endothelin-1 genetics MeSH
- Gene Frequency MeSH
- Genetic Predisposition to Disease MeSH
- Genotype MeSH
- Middle Aged MeSH
- Humans MeSH
- Lymphotoxin-alpha genetics MeSH
- Polymerase Chain Reaction MeSH
- Polymorphism, Genetic * MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Peptidyl-Dipeptidase A MeSH
- DNA Primers MeSH
- Endothelin-1 MeSH
- Lymphotoxin-alpha MeSH
The object of the study was to investigate the share of the polymorphisms I/D ACE, endothelin 1 4127G/A and TNF-beta NcoI in the susceptibility to proliferative diabetic retinopathy (PDR) in non-insulin-dependent diabetes mellitus (NIDDM). Genotypes were detected by polymerase chain reactions and determined in a set of 246 Caucasian NIDDM subjects with defined PDR status. The relevance of genotypes and clinical characteristics to the PDR occurrence was tested using multiple linear regression models and discrimination analysis. The best predictive value for PDR was given by a combination of two parameters - NIDDM duration and the TNF-beta genotype (p < 1.10(-6) and p = 1.10(-2), respectively) with a correct retrograde prediction of 82.6%. A comparison of the TNF-beta NcoI allele frequencies revealed no difference between NIDDM and nondiabetic subjects (n = 176), but a statistically significant difference was found between PDR and non-PDR NIDDM subjects (after a correction for the number of comparisons p = 0.03), allele beta2 being associated with PDR. Our results identified the allele variant TNF-beta2 being associated with PDR in NIDDM. Diabetes duration and the TNF-beta NcoI genotype were proven to significantly predict PDR occurrence. The TNF-beta2 allele could be regarded as a separate genetic risk factor that increases the relative incidence of PDR in patients with NIDDM.
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