The restriction-modification system in Streptomyces flavopersicus
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11501397
DOI
10.1007/bf02873588
Knihovny.cz E-resources
- MeSH
- DNA Restriction-Modification Enzymes metabolism MeSH
- Escherichia coli genetics MeSH
- Cloning, Molecular MeSH
- DNA Methylation MeSH
- Plasmids MeSH
- DNA, Recombinant metabolism MeSH
- Streptomyces enzymology genetics MeSH
- Transformation, Genetic MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA Restriction-Modification Enzymes MeSH
- DNA, Recombinant MeSH
To clone bifunctional vectors in streptomycetes, it was necessary to define the restriction-modification system of Streptomyces flavopersicus. Plasmid DNA from bifunctional vectors pIJ699 and pXED3-13, isolated from E. coli strains with different methylation systems: E. coli DH5 alpha (dam+ dcm+), E. coli MB5386 (dam dcm), E. coli CB51 (dam dcm+), E. coli NM544 (dam+ dcm), was used for transformation of protoplasts from strain S. flavopersicus. Restriction of dcm-methylated DNA from S. flavopersicus was established. As a host in the intermediate cloning strain E. coli NM544 (dam+ dcm) should be used, as the dcm-transmethylase-dependent strain S. flavopersicus does not process DNA from this strain.
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