Comparison of laser-Doppler flowmetry with biochemical indicators of endothelial dysfunction related to early microangiopathy in Type 1 diabetic patients
Language English Country United States Media print
Document type Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
11522496
DOI
10.1016/s1056-8727(01)00152-0
PII: S1056-8727(01)00152-0
Knihovny.cz E-resources
- MeSH
- beta-N-Acetyl-Galactosaminidase MeSH
- Biomarkers blood MeSH
- Endothelium, Vascular physiopathology MeSH
- Diabetes Mellitus, Type 1 physiopathology MeSH
- Diabetic Angiopathies physiopathology MeSH
- Adult MeSH
- E-Selectin blood MeSH
- Hexosaminidases blood MeSH
- Hyperemia etiology physiopathology MeSH
- Constriction MeSH
- Laser-Doppler Flowmetry standards MeSH
- Middle Aged MeSH
- Humans MeSH
- Intercellular Adhesion Molecule-1 blood MeSH
- Microcirculation MeSH
- Forearm blood supply MeSH
- Reference Values MeSH
- Hot Temperature MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- beta-N-Acetyl-Galactosaminidase MeSH
- Biomarkers MeSH
- E-Selectin MeSH
- Hexosaminidases MeSH
- Intercellular Adhesion Molecule-1 MeSH
The aim of this study was to compare biochemical markers of endothelial activation with microcirculation measured by laser-Doppler flowmetry in Type 1 diabetic patients with or without microangiopathy. A total of 44 Type 1 diabetic patients were subdivided into those with (n=24) and without (n=20) microangiopathy according to ophthalmological findings and the presence or absence of microalbuminuria. The control group consisted of 25 healthy people of comparable age, sex, and body mass index. Postocclusive reactive hyperemia (PORH) and thermal hyperemia (TH, at 44 degrees C) were measured at the forearm. Serum N-acetyl-beta-glucosaminidase (NAG) activity, serum E-selectin, and ICAM-1 concentrations were used as biochemical markers of endothelial dysfunction. A significantly lower velocity of perfusion increase during postocclusive hyperemia (PORH(max) x t(1)(-1)) and during thermal hyperemia (TH(max) x t(2)(-1)) (P<.01) were accompanied by higher serum NAG activity (20.9+/-4.6 vs. 16.3+/-2.5 U l(-1), P<.01) in diabetic patients with microangiopathy as compared to healthy persons. An inverse relationship was found between PORH(max) x t(1)(-1) and NAG (r=-.33) results in diabetic patients. In addition, higher mean values of serum NAG activity, E-selectin, and ICAM-1 concentrations were associated with significantly lower values of microcirculation parameters (PORH(max) x t(2)(-1) and TH(max) x t(2)(-1)) in six patients without microangiopathy who had at least one of the above biochemical markers higher than mean+2 S.D. range. We suggest that serum NAG activity, E-selectin, and ICAM-1 concentrations may be used together with laser-Doppler flowmetry in Type 1 diabetic patients as early indicators of vascular changes in very early stage of diabetic microangiopathy.
References provided by Crossref.org
Oxidative stress and endothelium influenced by metformin in type 2 diabetes mellitus
