Lectin histochemistry of microvascular endothelium in chick and quail musculature
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11789988
DOI
10.1007/s004290100212
Knihovny.cz E-resources
- MeSH
- Endothelium, Vascular chemistry metabolism MeSH
- Chimera MeSH
- Immunoenzyme Techniques MeSH
- Extremities embryology transplantation MeSH
- Muscle, Skeletal blood supply embryology MeSH
- Quail embryology MeSH
- Chick Embryo physiology MeSH
- Lectins analysis metabolism MeSH
- Microcirculation MeSH
- Plant Lectins MeSH
- Binding Sites MeSH
- Animals MeSH
- Check Tag
- Chick Embryo physiology MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Lectins MeSH
- Plant Lectins MeSH
The lectin binding pattern of muscular microvessels in chick, quail and chick/quail chimeras was analysed. Paraffin wax sections of muscles from embryonic and adult animals were used. The biotin-labelled lectins were detected by avidin-alkaline phosphatase complex. The following lectins bound to muscular microvessels including arterioles, capillaries and venules of both species: SNA-I (Sambucus nigra agglutinin), MAA (Maackia amurensis agglutinin), AIA (Artocarpus integrifolia agglutinin), VAA-I, VAA-II and VAA-III (Viscum album agglutinin I-III), WGA (wheat germ agglutinin), LEA (Lycopersicon esculentum agglutinin). Endomysium and basement membranes of muscle fibres were also stained to a variable extent and intensity. Only SNA-I stained almost exclusively the endothelium of blood vessels. WFA (Wisteria floribunda agglutinin) bound to the quail endothelium only. MPA (Maclura pomifera agglutinin) marked vessels in adult muscles of chick and quail, but embryonic vessels were stained in quail only. Our results show that lectin histochemistry is a useful tool for visualisation of microvasculature in avian species. In particular, WFA and MPA can be used to determine the origin of endothelia in chick/quail chimeras.
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