The effect of two abiotic elicitors--two newly synthesized compounds (substituted anilides of pyrazine-2-carboxylic acids)--on the formation of flavonoids in the callus culture of Ononis arvensis L. was tested. The compounds markedly influenced the production of flavonoids in an in vitro culture. Particularly after elicitation with a solution of compound No. 1 (4-hydroxyanilide 6-chloro-5-terc.butylpyrazine-2-carboxylic acid) in a concentration of 3.32.10(-7) mol.l-1 and within 48 hours of elicitation an increase in flavonoid formation by 976% versus the control took place.

Katedra farmakognozie Farmaceutické fakulty Univerzity Karlovy Hradec Králové

Substituted pyrazinecarboxamides as abiotic elicitors of flavolignan production in Silybum marianum (L.) gaertn cultures in vitro

Substituted N-phenylpyrazine-2-carboxamides, their synthesis and evaluation as herbicides and abiotic elicitors