We developed a nested PCR assay that detects the recA gene of the Burkholderia cepacia complex in sputum. The product of the first PCR round is also used to identify the genomovar of the pathogen. The protocol achieves high sensitivity and specificity with simple interpretation of genomovar status.

2nd Department of Pediatrics 2nd Medical School of Charles University Prague Czech Republic

J Clin Microbiol 2002 Dec;40(12):4806 PubMed

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Direct culture-independent Strain typing of Burkholderia cepacia complex in sputum samples from patients with cystic fibrosis

Differentiation of species combined into the Burkholderia cepacia complex and related taxa on the basis of their fatty acid patterns