Streptomycetes cultured on glass beads: Sample preparation for SEM
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12203711
DOI
10.1002/jemt.10126
Knihovny.cz E-resources
- MeSH
- Bacteriological Techniques * MeSH
- Microscopy, Electron, Scanning methods MeSH
- Glass MeSH
- Streptomyces growth & development ultrastructure MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
We demonstrate the preparation of samples of streptomycetes (Streptomyces coelicolor, S. aureofaciens) cultured on glass beads (balotina) for scanning electron microscopy. The main trick of the method consists in immobilization of glass beads with low-melting agarose. The samples are then fixed in OsO(4) vapors followed by dehydration in vapors of absolute ethanol. No air-to-liquid transition during the sample preparation occurs. Consequently, whole cell cycle of streptomycetes in the term of mycelial morphology can readily be studied by this method.
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