Cultivation system using glass beads immersed in liquid medium facilitates studies of Streptomyces differentiation
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
15932976
PubMed Central
PMC1151819
DOI
10.1128/aem.71.6.2848-2852.2005
PII: 71/6/2848
Knihovny.cz E-zdroje
- MeSH
- 2D gelová elektroforéza MeSH
- bakteriální proteiny metabolismus MeSH
- bakteriologické techniky MeSH
- kultivační média MeSH
- mikroskopie elektronová rastrovací MeSH
- proteomika MeSH
- regulace genové exprese u bakterií MeSH
- sklo * MeSH
- Streptomyces růst a vývoj metabolismus ultrastruktura MeSH
- velikost částic MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bakteriální proteiny MeSH
- kultivační média MeSH
A two-phase cultivation system was developed which will enable studies of streptomycete differentiation by molecular biological and global techniques such as transcriptomics and proteomics. The system is based on a solid phase formed by glass beads corresponding to particles in soil, clay, or sand natural habitats of streptomycetes. The beads are immersed in a liquid medium that allows easy modification or replacement of nutrients and growth factors as well as radioactive labeling of proteins. Scanning electron microscopy was used to analyze morphological differentiation of streptomycetes on glass beads and two-dimensional protein electrophoresis to demonstrate the potential of the system for analyses of protein synthesis profiles during the developmental program. This system facilitates studies of differentiation including expression and post-translation modifications of streptomycetes proteins, secondary metabolite biosynthesis, and morphological development.
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