Neonatal rat cardiomyocytes--a model for the study of morphological, biochemical and electrophysiological characteristics of the heart
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
12426771
Knihovny.cz E-resources
- MeSH
- Electrophysiology MeSH
- Myocytes, Cardiac physiology MeSH
- Rats physiology MeSH
- Myocardium cytology metabolism MeSH
- Animals, Newborn physiology MeSH
- Heart physiology MeSH
- Animals MeSH
- Check Tag
- Rats physiology MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
The neonatal rat cardiomyocyte model enables heart researchers to study and understand the morphological, biochemical and electrophysiological characteristics of the heart. This model offers a broad spectrum of experiments, such as studies of contraction, ischaemia, hypoxia and the toxicity of various compounds. This review examines the methodology for the isolation and cultivation of primary neonatal rat cardiomyocyte cultures, considers possible problems and pitfalls, and presents an optimized protocol for cardiomyocyte preparation.
