Salivary gland extract from Ixodes ricinus tick modulates the host immune response towards the Th2 cytokine profile
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- CD3 Complex metabolism MeSH
- Antigens, Differentiation, B-Lymphocyte metabolism MeSH
- Cell Extracts pharmacology MeSH
- Antigens, CD metabolism MeSH
- Cytokines analysis biosynthesis classification MeSH
- Macrophage Migration-Inhibitory Factors analysis biosynthesis MeSH
- Host-Parasite Interactions MeSH
- Interleukin-1 biosynthesis classification MeSH
- Ixodes chemistry cytology physiology MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Mitogens pharmacology MeSH
- Mice MeSH
- Flow Cytometry MeSH
- Receptors, Transferrin MeSH
- Spleen cytology growth & development MeSH
- Salivary Glands chemistry cytology MeSH
- Th1 Cells immunology MeSH
- Th2 Cells immunology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- CD3 Complex MeSH
- Antigens, Differentiation, B-Lymphocyte MeSH
- Cell Extracts MeSH
- Antigens, CD MeSH
- CD71 antigen MeSH Browser
- Cytokines MeSH
- Macrophage Migration-Inhibitory Factors MeSH
- Interleukin-1 MeSH
- Mitogens MeSH
- Receptors, Transferrin MeSH
In our previous work, the salivary gland extract (SGE) from Ixodes ricinus ticks impaired T-lymphocyte proliferation and clearly modulated the immune response towards the Th2 pattern in human peripheral blood mononuclear cell culture. In the present work, the results obtained on mouse splenocytes are compared with those on human leukocytes. ELISA (protein level) and RNAse protection assay (mRNA level) showed that SGE enhanced interleukin (IL)-1alpha, IL-1beta, IL-1Ra, IL-6, and IL-12p40 cytokines, whereas production of IL-2, IL-5, IL-10, and IL-13 was decreased. The minute levels of IL-9, IL-15 and IL-12p70 were not changed after the addition of tick saliva. IL-4 was upregulated, whereas the production of gamma interferon and migratory inhibition factor was downregulated after the addition of SGE. Tick saliva decreased concanavalin A-stimulated spleen cell proliferation and the percentage of activated T-cells. We conclude that the Th2 polarization did not involve all of the cytokines tested. However, the Th2 subset-augmenting effect of tick saliva was confirmed.
References provided by Crossref.org
Modulation of host immunity by tick saliva
Insight into the sialome of the castor bean tick, Ixodes ricinus
Tick saliva in anti-tick immunity and pathogen transmission
