Cíl: Cílem naší studie bylo kvantifikovat a porovnat imunobarvení IL-2, IL-5, IL-6, IL-8 a TNFa ve tkáni endometriomu, nenádorových nádorech, benigních novotvarech a maligních novotvarech vaječníků. Materiál a metody: Studie se zúčastnilo 90 pacientek; bylo dia gnostikováno 15 nenádorových lézí ovaria, 28 ovariálních benigních novotvarů, 28 ovariálních maligních novotvarů a 19 ovariálních endometriomů. Imunohistochemie byla provedena pro cytokiny IL-2, IL-5, IL-6, IL-8 a TNFa a jejich koncentrace byly v těchto skupinách porovnány. Použili jsme Fisherův exaktní test, podle něhož jsou významné hodnoty p < 0,05. Výsledky: Imunobarvení epitelu na přítomnost IL-5 a IL-8 je silnější u endometriomů než u karcinomu vaječníků (p hodnoty 0,0046 a 0,0149). Stromální imunobarvení TNFa, IL-5, IL-6 a IL8 je silnější u endometriomů než u rakoviny vaječníků (p hodnoty 0,0008; < 0,0001; 0,0003 a 0,0006). Závěr: Silnější imunobarvení ně kte rých cytokinů u endometriomů ve srovnání s karcinomem ovaria odráží zánětlivou a imunitní odpověď, která by mohla být budoucím cílem nových objevů o infiltrativním chování endometriózy.
Objective: The objective of our study was to quantify and compare the immunostaining of IL-2, IL-5, IL-6, IL-8, and TNF-α in endometriomal tissue, non-neoplastic tumors, benign neoplasms, and malignant ovarian neoplasms. Materials and methods: The study involved 90 patients: 15 non-neoplastic ovarian lesions, 28 ovarian benign neoplasms, 28 ovarian malignant neoplasms, and 19 ovarian endometriomas were diagnosed. Immunohistochemistry was performed for cytokines IL-2, IL-5, IL-6, IL-8, and TNF-α and their concentrations were compared in these groups. Fisher‘s exact test was used, requiring a P-value of < 0.05 for significance. Results: IL-5 and IL-8 epithelial immunostaining is stronger in endometriomas than in ovarian cancer (P-values of 0.0046 and 0.0149, resp.). The stromal immunostaining of TNF-α, IL-5, IL-6, and IL8 is stronger in endometriomas than in ovarian cancer (P-values of 0.0008, < 0.0001, 0.0003, and 0.0006, resp.). Conclusions: Stronger immunostaining of some cytokines in endometriomas compared to ovarian cancer reflects an inflammatory and immune response that could be future targets for new discoveries about the infiltrative behavior of endometriosis.
- MeSH
- Staining and Labeling classification methods MeSH
- Cytokines analysis immunology classification MeSH
- Endometriosis * diagnostic imaging diagnosis immunology MeSH
- Immunohistochemistry * classification methods MeSH
- Interleukin-6 analysis immunology MeSH
- Humans MeSH
- Ovarian Neoplasms * diagnostic imaging immunology classification MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Clinical Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- MeSH
- Cytokines physiology classification MeSH
- Inflammasomes physiology MeSH
- Humans MeSH
- Lipids * biosynthesis physiology classification MeSH
- Receptors, Pattern Recognition classification MeSH
- Hypothalamo-Hypophyseal System physiology MeSH
- Inflammation * physiopathology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Review MeSH
- MeSH
- Cytokines * pharmacology immunology classification therapeutic use MeSH
- Erythropoietin analogs & derivatives pharmacology classification therapeutic use MeSH
- Granulocyte Colony-Stimulating Factor pharmacology immunology classification therapeutic use MeSH
- Interferons pharmacology immunology classification therapeutic use MeSH
- Interleukin-1 pharmacology immunology therapeutic use MeSH
- Interleukin-2 pharmacology immunology therapeutic use MeSH
- Receptors, Cytokine immunology classification MeSH
- Thrombopoietin agonists pharmacology classification therapeutic use MeSH
- Tumor Necrosis Factor-alpha pharmacology therapeutic use MeSH
- Publication type
- Review MeSH
- MeSH
- Semen Analysis methods MeSH
- Annexins antagonists & inhibitors immunology MeSH
- Antibodies, Antinuclear immunology MeSH
- Autoantibodies analysis immunology classification MeSH
- Killer Cells, Natural physiology immunology MeSH
- Cytokines analysis classification MeSH
- Immunologic Tests * classification methods MeSH
- Humans MeSH
- Reproduction * immunology MeSH
- Spermatozoa immunology MeSH
- T-Lymphocytes immunology classification MeSH
- Cell Migration Assays, Leukocyte methods MeSH
- Transglutaminases antagonists & inhibitors immunology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Review MeSH
- Keywords
- adsorpční kolona, TheraSorb Life 18,
- MeSH
- Dermatitis, Atopic * pathology therapy MeSH
- Cytokines physiology classification MeSH
- Child MeSH
- Adult MeSH
- Immunoglobulin E blood MeSH
- Immunosorbent Techniques * instrumentation MeSH
- Job Syndrome pathology MeSH
- Keratinocytes pathology MeSH
- Quality of Life MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Plasmapheresis * classification methods instrumentation MeSH
- Th2 Cells MeSH
- Treatment Outcome MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Male MeSH
- Female MeSH
- MeSH
- Adaptive Immunity physiology MeSH
- Hypersensitivity immunology classification MeSH
- Cytokines physiology immunology classification MeSH
- Dendritic Cells physiology immunology MeSH
- Immunity * MeSH
- Immune System physiology immunology MeSH
- Immunoglobulins analysis classification MeSH
- Interferon Type I analysis immunology MeSH
- Complement System Proteins analysis immunology MeSH
- Leukocytes physiology immunology MeSH
- Humans MeSH
- Lymphocytes physiology immunology MeSH
- Macrophages physiology immunology MeSH
- Mast Cells immunology MeSH
- Immunity, Innate physiology MeSH
- Rheumatic Diseases * immunology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Review MeSH
- MeSH
- Immunotherapy, Active MeSH
- CTLA-4 Antigen drug effects MeSH
- Programmed Cell Death 1 Receptor drug effects MeSH
- Cytokines immunology classification MeSH
- Dendritic Cells immunology classification MeSH
- Vaccines, DNA MeSH
- Immune System MeSH
- Immunomodulation * immunology drug effects MeSH
- Immunotherapy, Adoptive MeSH
- Immunotherapy * classification methods utilization MeSH
- Ipilimumab MeSH
- Medical Oncology * methods manpower trends MeSH
- Humans MeSH
- Melanoma diagnosis drug therapy complications MeSH
- Neoplasm Metastasis diagnosis drug therapy MeSH
- Antibodies, Monoclonal administration & dosage pharmacology therapeutic use MeSH
- Kidney Neoplasms diagnosis drug therapy complications MeSH
- Carcinoma, Non-Small-Cell Lung diagnosis drug therapy complications MeSH
- Nivolumab MeSH
- Nobel Prize MeSH
- Check Tag
- Humans MeSH
- Publication type
- Introductory Journal Article MeSH
- Keywords
- membránové molekuly,
- MeSH
- Chemokines agonists physiology classification MeSH
- Cytokines agonists physiology classification MeSH
- Immune System Phenomena * physiology MeSH
- Integrins classification MeSH
- Humans MeSH
- Cell Adhesion Molecules physiology classification MeSH
- Antibodies, Monoclonal MeSH
- Receptors, Chemokine physiology classification MeSH
- Receptors, Cytokine classification MeSH
- Selectins physiology MeSH
- Signal Transduction physiology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Review MeSH
Mnohočetný myelom (MM) a Waldenströmova makroglobulinemie (WM) jsou nádorová onemocnění B lymfocytů, pro která je charakteristická nadprodukce monoklonálního imunoglobulinu a infiltrace kostní dřeně (KD) maligními buňkami. Tyto patologické jevy vedou posléze k typickým klinickým příznakům onemocnění, především ke vzniku osteolytických lézí a poruchám krvetvorby. U obou těchto onemocnění hraje velmi významnou roli mikroprostředí KD, jelikož interakce nádorových buněk s buňkami KD usnadňuje růst a přežívání těchto maligních buněk. Tyto interakce jsou zprostředkovány mimo jiné také cytokiny. Jejich produkce umožňuje růst, proliferaci a přežívání nádorových buněk, a tak významně přispívají k patogenezi MM i WM. V předkládaném přehledovém článku jsme se zaměřili na funkci nejdůležitějších cytokinů u obou onemocnění.
Multiple myeloma (MM) and Waldenström macroglobulinemia (WM) are malignant disorders of B lymphocytes. These diseases are characterized by monoclonal immunoglobulin production and bone marrow infiltration, which further lead to disease manifestation mainly via osteolytic lesions and disruption of hematopoiesis. The bone marrow microenvironment plays a crucial role in pathogenesis of both of these diseases, as it is well known that interaction between malignant cells and bone marrow cells facilitates both survival and growth of these tumor cells. The interactions are mediated by several different factors, including cytokines. Their production leads to tumor cell growth, proliferation and survival contributing to pathogenesis of MM and WM. In this review, we focus on function of the most important cytokines in both these diseases. Key words: multiple myeloma − Waldenström macroglobulinemia – cytokines − bone marrow microenvironment This study was supported by scientific programs of the Ministry of Education, Youth and Sports MSM0021622434, by grant of Integral Grant Agency of the Czech ministry of Health NT14575 and NT12130 and by internal grant MUNI/11//InGA17/2012. Further it was supported by project of the Ministry of Health for conceptual development of research organization 65269705 (UH Brno). The authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study. The Editorial Board declares that the manuscript met the ICMJE “uniform requirements” for biomedical papers. Submitted: 5. 9. 2013 Accepted: 7. 11. 2013
- MeSH
- Chemokine CCL3 metabolism MeSH
- Cytokines * diagnostic use classification metabolism MeSH
- Adult MeSH
- B-Cell Activating Factor immunology metabolism MeSH
- Hepatocyte Growth Factor diagnostic use metabolism MeSH
- Insulin-Like Growth Factor I metabolism MeSH
- Interleukin-6 diagnostic use physiology metabolism MeSH
- Bone Marrow * immunology pathology MeSH
- Humans MeSH
- RANK Ligand metabolism MeSH
- Multiple Myeloma * diagnosis blood physiopathology MeSH
- NF-kappa B MeSH
- Osteoprotegerin immunology MeSH
- Aged MeSH
- Vascular Endothelial Growth Factors physiology metabolism MeSH
- Waldenstrom Macroglobulinemia diagnosis blood physiopathology MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Aged MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
