Speciation of Cu, Se, Zn and Fe in blood serum of hemodialysed patients
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
12448934
Knihovny.cz E-zdroje
- MeSH
- dialýza ledvin * MeSH
- lidé MeSH
- měď krev MeSH
- selen krev MeSH
- stopové prvky krev MeSH
- železo krev MeSH
- zinek krev MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- měď MeSH
- selen MeSH
- stopové prvky MeSH
- železo MeSH
- zinek MeSH
The speciation of trace elements in serum samples of hemodialysed patients was investigated using on-line connection of SEC and ICP-MS. The 0.02 mol/l TRIS-HCl buffer of pH 7.5 was used as mobile phase. The results of speciation as well as the total concentration data were compared with those of control group of healthy person. Alterations of total concentration were observed in case of selenium and zinc only. Iron was present in form of transferrin and ferritin, main amount of copper was bound to ceruloplasmin and selenium compounds were identified as selenoproteine P and glutathione peroxidase. The latter compound was detected in samples of control group only. The chromatograms of the other elements were similar and no substantial changes between both investigated groups were observed.
