Water ADC, extracellular space volume, and tortuosity in the rat cortex after traumatic injury
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
12465109
DOI
10.1002/mrm.10305
Knihovny.cz E-zdroje
- MeSH
- difuze MeSH
- extracelulární prostor metabolismus MeSH
- gangliová stimulancia farmakologie MeSH
- glióza patologie MeSH
- imunohistochemie MeSH
- krysa rodu Rattus MeSH
- magnetická rezonanční spektroskopie MeSH
- magnetická rezonanční tomografie * MeSH
- mapování mozku MeSH
- modely nemocí na zvířatech MeSH
- mozková kůra patologie patofyziologie MeSH
- poranění mozku diagnóza metabolismus MeSH
- potkani Wistar MeSH
- senzitivita a specificita MeSH
- voda metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- gangliová stimulancia MeSH
- voda MeSH
The diffusion parameters in rat cortex were studied 3-35 days following a cortical stab wound, using diffusion-weighted MR to determine the apparent diffusion coefficient of water (ADC(W)) in the tissue, and the real-time iontophoretic tetramethylammonium (TMA) method to measure the extracellular space (ECS) diffusion parameters: ECS volume fraction alpha and the ADC of TMA(+) (ADC(TMA)). Severe astrogliosis was found close to the wound, and mild astrogliosis was found in the ipsilateral but not the contralateral cortex. Chondroitin sulfate proteoglycan (CSPG) expression was increased throughout the ipsilateral cortex. In the hemisphere contralateral to the wound, alpha, ADC(TMA), and ADC(W) were not significantly different from control values. ECS volume fraction was increased only in the vicinity of the wound, in the region of cell death and severe astrogliosis, at 3 and 7 days after injury. However, both ADC(TMA) and ADC(W) were significantly decreased after lesion in the vicinity of the wound as well as in the rest of the ipsilateral hemisphere distant from the wound. Thus, both ADC(W) and ADC(TMA) decreased in regions wherein alpha did not change but CSPG increased. An increase in extracellular matrix expression may therefore impose diffusion barriers for water as well as for TMA molecules.
Citace poskytuje Crossref.org
Astrocytes and extracellular matrix in extrasynaptic volume transmission
Diffusion in brain extracellular space
