Water ADC, extracellular space volume, and tortuosity in the rat cortex after traumatic injury
Language English Country United States Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
12465109
DOI
10.1002/mrm.10305
Knihovny.cz E-resources
- MeSH
- Diffusion MeSH
- Extracellular Space metabolism MeSH
- Ganglionic Stimulants pharmacology MeSH
- Gliosis pathology MeSH
- Immunohistochemistry MeSH
- Rats MeSH
- Magnetic Resonance Spectroscopy MeSH
- Magnetic Resonance Imaging * MeSH
- Brain Mapping MeSH
- Disease Models, Animal MeSH
- Cerebral Cortex pathology physiopathology MeSH
- Brain Injuries diagnosis metabolism MeSH
- Rats, Wistar MeSH
- Sensitivity and Specificity MeSH
- Water metabolism MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Ganglionic Stimulants MeSH
- Water MeSH
The diffusion parameters in rat cortex were studied 3-35 days following a cortical stab wound, using diffusion-weighted MR to determine the apparent diffusion coefficient of water (ADC(W)) in the tissue, and the real-time iontophoretic tetramethylammonium (TMA) method to measure the extracellular space (ECS) diffusion parameters: ECS volume fraction alpha and the ADC of TMA(+) (ADC(TMA)). Severe astrogliosis was found close to the wound, and mild astrogliosis was found in the ipsilateral but not the contralateral cortex. Chondroitin sulfate proteoglycan (CSPG) expression was increased throughout the ipsilateral cortex. In the hemisphere contralateral to the wound, alpha, ADC(TMA), and ADC(W) were not significantly different from control values. ECS volume fraction was increased only in the vicinity of the wound, in the region of cell death and severe astrogliosis, at 3 and 7 days after injury. However, both ADC(TMA) and ADC(W) were significantly decreased after lesion in the vicinity of the wound as well as in the rest of the ipsilateral hemisphere distant from the wound. Thus, both ADC(W) and ADC(TMA) decreased in regions wherein alpha did not change but CSPG increased. An increase in extracellular matrix expression may therefore impose diffusion barriers for water as well as for TMA molecules.
References provided by Crossref.org
Astrocytes and extracellular matrix in extrasynaptic volume transmission
Diffusion in brain extracellular space
