Age- and sex-dependent effects of ethanol on hippocampal hemicholinium-3 sensitive choline carriers during postnatal development of rats
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Anisotropy MeSH
- Biological Transport drug effects MeSH
- Time Factors MeSH
- Choline pharmacokinetics MeSH
- Cholinergic Agents metabolism MeSH
- Ethanol administration & dosage pharmacology MeSH
- Hemicholinium 3 metabolism MeSH
- Hippocampus metabolism MeSH
- Binding, Competitive drug effects MeSH
- Rats MeSH
- Central Nervous System Depressants administration & dosage pharmacology MeSH
- Lipid Bilayers metabolism MeSH
- Membrane Transport Proteins drug effects metabolism MeSH
- Animals, Newborn growth & development metabolism MeSH
- Sex Characteristics * MeSH
- Rats, Wistar MeSH
- Aging metabolism MeSH
- Binding Sites drug effects MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Choline MeSH
- choline transporter MeSH Browser
- Cholinergic Agents MeSH
- Ethanol MeSH
- Hemicholinium 3 MeSH
- Central Nervous System Depressants MeSH
- Lipid Bilayers MeSH
- Membrane Transport Proteins MeSH
Vulnerability of hippocampal hemicholinium-3 (HC-3)-sensitive carriers to ethanol was evaluated in vitro during rat postnatal development. The high-affinity uptake of [3H]choline (HACU) and the specific binding of [3H]HC-3 were measured on synaptosomes from 7-, 14-, and 60-day-and 3-month-old male and female Wistar rats. Marked increases of basal (between 7 and 60 days of age) and of stimulated HACU levels via K(+)-depolarization (between 14 days and 3 months) but only a mild elevation in [3H]HC-3 binding (between 7 days and 3 months) associated with alterations in the binding site number were found. On the mature tissue, ethanol at high concentrations (5%) moderately inhibited the choline transport under basal conditions but totally eliminated depolarization effects. However, both age- and sex-dependent alterations in basal HACU mediated by high or low pharmacologically relevant alcohol concentrations (50-100 mM) were observed in the immature tissue. Namely, the dose- and incubation time-dependent inhibition of HACU associated with changes in the transport velocity was found in postnatal male but not female tissue. [3H]HC-3 binding site was not markedly sensitive to ethanol actions. Anisotropy measurements in the region of the hydrophilic heads of phospholipid bilayers and in the membrane hydrocarbon core indicated penetration of 100 mM ethanol to immature female but not male tissue. Our results suggest the noncompetitive binding of alcohol to choline carriers from immature male tissue and correspond with data reporting significant sexual dimorphism of postnatal hippocampal neurons. The direct effects of ethanol on male choline carriers can contribute to the inhibition of acetylcholine synthesis and to sex-dependent neurotoxic effects of alcohol applied in vivo during early and late postnatal period.
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Brain Res. 1993 Jan 22;601(1-2):221-9 PubMed
Brain Res Dev Brain Res. 1991 Sep 19;62(1):51-60 PubMed
J Neurochem. 1998 Sep;71(3):1095-107 PubMed
Eur J Pharmacol. 1988 Jun 22;151(1):51-8 PubMed
Brain Res. 1987 Oct;432(2):175-81 PubMed
Brain Res. 1999 Jan 9;815(2):358-66 PubMed
Biochem Biophys Res Commun. 2000 Oct 5;276(3):862-7 PubMed
Neurochem Res. 1995 Aug;20(8):939-49 PubMed
Alcohol Clin Exp Res. 1991 Feb;15(1):136-40 PubMed
J Neurochem. 1985 Jan;44(1):11-24 PubMed
J Hirnforsch. 1986;27(1):5-17 PubMed
J Pharmacol Exp Ther. 1990 Sep;254(3):1029-37 PubMed
J Neurochem. 1976 May;26(5):909-22 PubMed
FEBS Lett. 2000 Nov 3;484(2):92-7 PubMed
Neuroscience. 1989;33(3):435-62 PubMed
Alcohol Clin Exp Res. 1985 Mar-Apr;9(2):164-80 PubMed
Brain Res. 2001 Jan 26;890(1):110-7 PubMed
J Neurosci. 1990 Jan;10 (1):62-72 PubMed
Nature. 1996 May 2;381(6577):71-5 PubMed
Ann N Y Acad Sci. 1991;627:264-76 PubMed
J Comp Neurol. 1992 Jul 22;321(4):591-611 PubMed
Neurochem Res. 1998 Jul;23(7):923-9 PubMed
Neurosci Lett. 1993 Jun 25;156(1-2):13-6 PubMed
Brain Res. 1995 Jul 10;685(1-2):21-32 PubMed
Alcohol Clin Exp Res. 1988 Dec;12(6):795-800 PubMed
Eur J Pharmacol. 1994 Feb 15;266(3):283-9 PubMed
Alcohol Clin Exp Res. 1995 Aug;19(4):840-5 PubMed
Neurochem Res. 2001 Mar;26(3):203-12 PubMed
Pharmacology. 1989;39(6):367-72 PubMed
Alcohol Clin Exp Res. 1988 Feb;12(1):179-83 PubMed
Alcohol Clin Exp Res. 1993 Dec;17(6):1215-22 PubMed
Alcohol Clin Exp Res. 1993 Jun;17(3):643-50 PubMed
Neurotoxicology. 1989 Fall;10(3):569-76 PubMed
Brain Res. 1975 Aug 29;94(2):325-36 PubMed
Anal Biochem. 1976 May 7;72:248-54 PubMed
Brain Res. 1979 Mar 16;163(2):263-75 PubMed
Brain Res. 1987 Aug;431(2):291-7 PubMed
Brain Res. 1976 Dec 24;118(3):429-40 PubMed
Dement Geriatr Cogn Disord. 2000 Jul-Aug;11(4):181-6 PubMed
J Neurochem. 1990 May;54(5):1500-8 PubMed
Brain Res Dev Brain Res. 1998 Feb 10;105(2):241-50 PubMed
J Neurochem. 1994 Oct;63(4):1328-37 PubMed
Alcohol Alcohol. 1991;26(5-6):605-13 PubMed
Epilepsia. 1999 Apr;40(4):414-23 PubMed
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