Preimplantacní genetická diagnostika (PGD) chromosomálních aberací metodou fluorescencní in situ hybridizace (FISH)--úvod do problematiky, prehled metod pri odbĕru materiálu a technika vysetrení
[Preimplantation genetic diagnosis (PGD) of chromosomal aberrations using the fluorescence in situ hybridization method (FISH)--introduction to problems, sampling methods and examination techniques]
Language Czech Country Czech Republic Media print
Document type English Abstract, Journal Article, Review
PubMed
12749176
- MeSH
- Chromosome Disorders diagnosis MeSH
- In Situ Hybridization, Fluorescence methods MeSH
- Humans MeSH
- Preimplantation Diagnosis methods MeSH
- Pregnancy MeSH
- Check Tag
- Humans MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- English Abstract MeSH
- Journal Article MeSH
- Review MeSH
OBJECTIVE: Presentation of preimplantation genetic diagnosis and the set of laboratory processes like aspiration, preparation and evaluation of polar bodies, sperm cells and blastomeres using FISH method (fluorescent in situ hybridization) in ART. DESIGN: Review. SETTING: Sanatorium REPROMEDA, Brno, Veterinary Research Institute, Brno. METHODS: Overview of published data and own clinical experience with the cell aspiration methods, evaluated sample preparation and the proper chromosomes visualisation using FISH method. CONCLUSION: The review brings an overview of conditions and methods including sample obtaining, FISH analysis preparation and implementation, processed during PGD.
