Digital imaging as a possible approach in evaluation of islet yield
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
- MeSH
- krysa rodu Rattus MeSH
- kultivované buňky MeSH
- Langerhansovy ostrůvky cytologie metabolismus MeSH
- počet buněk MeSH
- počítačové zpracování obrazu metody MeSH
- potkani Wistar MeSH
- separace buněk MeSH
- statistika jako téma MeSH
- velikost buňky MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
Digital image analysis (DIA) is a new method in assessment of islet amount, which is expected to provide reliable and consistent results. We compared this method with conventional counting of small numbers of rat islets. Islets were isolated from 8 pancreases and counted in 24 samples in duplicate, first routinely by sizing according to estimated diameters under a calibrated reticule and then by processing of islets pictures taken by camera. As presumed, no significant difference was found in absolute numbers of islets per sample between DIA and conventional assessment. Volumes of islets per sample measured by DIA were on average more than 10% higher than amounts evaluated conventionally, which was statistically significant. DIA has been shown to be an important method to remove operator bias and provide consistent results. Evaluation of only two dimensions of three-dimensional objects still represents a certain limitation of this technique. With lowering of computer prices the system could become easily available for islet laboratories.
Citace poskytuje Crossref.org
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