Passing of fluorescein derivatives into the hyphae of Phanerochaete chrysosporium
Language English Country United States Media print
Document type Journal Article
PubMed
12800503
DOI
10.1007/bf02930956
Knihovny.cz E-resources
- MeSH
- Enzyme Induction MeSH
- Fluorescein chemistry metabolism MeSH
- Fluorescent Dyes chemistry metabolism MeSH
- Hyphae metabolism MeSH
- Culture Media MeSH
- Peroxidases metabolism MeSH
- Phanerochaete growth & development metabolism MeSH
- Polyethylene Glycols chemistry pharmacology MeSH
- Polysorbates pharmacology MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Fluorescein MeSH
- Fluorescent Dyes MeSH
- Culture Media MeSH
- lignin peroxidase MeSH Browser
- Peroxidases MeSH
- Polyethylene Glycols MeSH
- Polysorbates MeSH
Fluorescein derivatives added into the growth medium were decolorized during submerged cultivation of Phanerochaete chrysosporium. The highest decrease of absorbance A450 was observed in the growth phase regardless of the presence of inducers Tween 80 or poly(ethylene glycol) (PEG). Fluorescein linked to PEG was prepared and, after addition to cultures, shown to stimulate the production of lignin peroxidase. Passing of fluorescing substances into hyphae (observed by confocal microscopy) showed that they were concentrated on some structures inside hyphae.
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