Production of manganese-dependent peroxidase in a new solid-state bioreactor by Phanerochaete chrysosporium grown on wood shavings. Application to the decolorization of synthetic dyes
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
12422521
DOI
10.1007/bf02818701
Knihovny.cz E-zdroje
- MeSH
- barva MeSH
- barvicí látky metabolismus MeSH
- biodegradace MeSH
- bioreaktory * MeSH
- dřevo MeSH
- kultivační média MeSH
- peroxidasy biosyntéza metabolismus MeSH
- Phanerochaete enzymologie růst a vývoj MeSH
- spektrofotometrie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- barvicí látky MeSH
- kultivační média MeSH
- lignin peroxidase MeSH Prohlížeč
- manganese peroxidase MeSH Prohlížeč
- peroxidasy MeSH
The production of manganese-dependent peroxidase (MnP) by Phanerochaete chrysosporium in a new solid-state bioreactor, the immersion bioreactor, operating with lignocellulosic waste, such as wood shavings, was investigated. Maximum MnP and lignin peroxidase (LiP) activity of 13.4 and 8.48 mukat/L were obtained, respectively. The in vitro decolorization of several synthetic dyes by the extracellular liquid produced in the above-mentioned bioreactor (containing mainly MnP) was carried out and its degrading ability was assessed. The highest decolorization was reached with Indigo Carmine (98%) followed by Bromophenol Blue (56%) and Methyl Orange (36%), whereas Gentian Violet was hardly decolorized (6%).
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