Improving the yield and quality of DNA isolated from white-rot fungi
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
15227780
DOI
10.1007/bf02931383
Knihovny.cz E-zdroje
- MeSH
- Basidiomycota chemie MeSH
- cetrimoniové sloučeniny chemie MeSH
- cetrimonium MeSH
- chitinasy metabolismus MeSH
- detergenty MeSH
- DNA fungální analýza izolace a purifikace metabolismus MeSH
- fenol chemie MeSH
- frakční precipitace MeSH
- mykologie metody MeSH
- Phanerochaete chemie MeSH
- Pleurotus chemie MeSH
- polysacharidy chemie MeSH
- restrikční enzymy metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- cetrimoniové sloučeniny MeSH
- cetrimonium MeSH
- chitinasy MeSH
- detergenty MeSH
- DNA fungální MeSH
- fenol MeSH
- polysacharidy MeSH
- restrikční enzymy MeSH
A new simple method used to eliminate polysaccharides that cause problems during DNA isolation was established for 6 different white-rot fungi using 1% hexadecyltrimethylammonium bromide (CTAB) as wash buffer and followed by centrifugation. Variation in the DNA yield and quality was ascertained using precipitating agents, detergents and cell-wall-hydrolyzing chitinase. Considerable amount of exopolysaccharides from fungal biomass was removed with the use of 1% CTAB wash buffer followed by centrifugation. The DNA varied in terms of yield and quality. For the DNA extraction use of 2% SDS in extraction buffer worked best for Pycnoporus cinnabarinus, Cyathus bulleri, Cyathus striatus and Cyathus stercoreus, while 2% CTAB worked best for Phanerochaete chrysosporium and Pleurotus ostreatus. Elimination of phenol and use of absolute ethanol for precipitating DNA resulted in good yield and quality of DNA. This DNA was amenable to restriction endonuclease digestion.
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Folia Microbiol (Praha). 2002;47(6):691-5 PubMed
Folia Microbiol (Praha). 2002;47(4):417-21 PubMed
Nucleic Acids Res. 1980 Oct 10;8(19):4321-5 PubMed
Biotechniques. 1997 Jan;22(1):47, 50-2 PubMed
J Gen Microbiol. 1978 Jun;106(2):387-9 PubMed
Folia Microbiol (Praha). 2002;47(4):385-90 PubMed
Biotechniques. 1991 Feb;10(2):162, 164, 166 PubMed
FEMS Microbiol Lett. 1990 Jan 1;54(1-3):113-7 PubMed
Anal Biochem. 1983 Dec;135(2):416-22 PubMed
Prep Biochem. 1995 Nov;25(4):171-81 PubMed
Anal Biochem. 1982 Jan 1;119(1):158-63 PubMed
Folia Microbiol (Praha). 2003;48(3):413-6 PubMed
Gene. 1997 Sep 1;196(1-2):113-9 PubMed
Annu Rev Microbiol. 1987;41:465-505 PubMed
Adv Biochem Eng Biotechnol. 1997;57:45-125 PubMed
Appl Environ Microbiol. 1999 Nov;65(11):4943-8 PubMed
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