Implication of Dichomitus squalens manganese-dependent peroxidase in dye decolorization and cooperation of the enzyme with laccase
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
- MeSH
- anthrachinony metabolismus MeSH
- azosloučeniny metabolismus MeSH
- bakteriální proteiny izolace a purifikace metabolismus MeSH
- barva MeSH
- barvicí látky metabolismus MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- lakasa izolace a purifikace metabolismus MeSH
- lignin metabolismus MeSH
- molekulová hmotnost MeSH
- mykologie metody MeSH
- naftalensulfonany metabolismus MeSH
- oxidace-redukce MeSH
- peroxidasy izolace a purifikace metabolismus MeSH
- Polyporaceae enzymologie MeSH
- pšenice MeSH
- synergismus léků MeSH
- trypanová modř metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- anthrachinony MeSH
- azosloučeniny MeSH
- bakteriální proteiny MeSH
- barvicí látky MeSH
- C.I. Reactive Violet 5 MeSH Prohlížeč
- lakasa MeSH
- lignin MeSH
- lignocellulose MeSH Prohlížeč
- manganese peroxidase MeSH Prohlížeč
- naftalensulfonany MeSH
- peroxidasy MeSH
- pontamine sky blue MeSH Prohlížeč
- reactive orange 16 MeSH Prohlížeč
- Remazol Brilliant Blue R MeSH Prohlížeč
- trypanová modř MeSH
Three new chromatographic forms of Dichomitus squalens manganese-dependent peroxidase (MnP) were isolated from wheat-straw cultures using Mono Q and connective interaction media (CIM) fast protein liquid chromatography. Enzymes revealed identical molar mass of 50 kDa (estimated by SDS-PAGE) and pI values of 3.5, however, they varied in Km values obtained for Mn2+ oxidation. The addition of wood and straw methanol extracts to the cultures showed that the production of MnPs in wheat-straw cultures was influenced rather by the type of cultivation than by phenolic compounds from lignocellulosic material which induced laccase production. The purified CIM1 MnP was able to decolorize selected azo and anthraquinone dyes more rapidly than laccase Lc1. In vitro dye decolorization showed a synergistic cooperation of MnP and laccase. In the case of CSB degradation MnP prevented from the production of a differently colored substance that could be produced after CSB degradation by laccase-HBT system.
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