2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein as a dual-emission fluorescent indicator of intracellular pH suitable for argon laser confocal microscopy
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
10983238
DOI
10.1007/bf02903718
Knihovny.cz E-zdroje
- MeSH
- argon MeSH
- fluoresceiny * MeSH
- fluorescenční barviva * MeSH
- fluorescenční spektrometrie MeSH
- intracelulární tekutina metabolismus MeSH
- koncentrace vodíkových iontů MeSH
- konfokální mikroskopie metody MeSH
- lasery MeSH
- průtoková cytometrie metody MeSH
- Saccharomyces cerevisiae metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein MeSH Prohlížeč
- argon MeSH
- fluoresceiny * MeSH
- fluorescenční barviva * MeSH
The widely used fluorescent probe 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) serves as a pH-sensitive indicator in classical microscopy. Characteristics of BCECF were studied and a way of employing the probe in a confocal laser scanning microscope equipped with an argon laser at 488 nm was developed, based on the fact that the emission fluorescence spectra are pH-dependent with spectral maximum shift from 518 to 529 nm. Optical filters for the dual-emission ratio method were set to 506 and 529 nm. pH values measured inside a single cell of Saccharomyces cerevisiae were similar to those obtained with other pH estimation methods.
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