2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein as a dual-emission fluorescent indicator of intracellular pH suitable for argon laser confocal microscopy
Language English Country United States Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
10983238
DOI
10.1007/bf02903718
Knihovny.cz E-resources
- MeSH
- Argon MeSH
- Fluoresceins * MeSH
- Fluorescent Dyes * MeSH
- Spectrometry, Fluorescence MeSH
- Intracellular Fluid metabolism MeSH
- Hydrogen-Ion Concentration MeSH
- Microscopy, Confocal methods MeSH
- Lasers MeSH
- Flow Cytometry methods MeSH
- Saccharomyces cerevisiae metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein MeSH Browser
- Argon MeSH
- Fluoresceins * MeSH
- Fluorescent Dyes * MeSH
The widely used fluorescent probe 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) serves as a pH-sensitive indicator in classical microscopy. Characteristics of BCECF were studied and a way of employing the probe in a confocal laser scanning microscope equipped with an argon laser at 488 nm was developed, based on the fact that the emission fluorescence spectra are pH-dependent with spectral maximum shift from 518 to 529 nm. Optical filters for the dual-emission ratio method were set to 506 and 529 nm. pH values measured inside a single cell of Saccharomyces cerevisiae were similar to those obtained with other pH estimation methods.
See more in PubMed
FEMS Microbiol Lett. 1995 Aug 1;130(2-3):245-51 PubMed
Exp Physiol. 1996 Mar;81(2):261-71 PubMed
Biochem Int. 1991 Jul;24(4):641-7 PubMed
Pflugers Arch. 1993 Feb;422(5):523-9 PubMed
FASEB J. 1996 Aug;10(10):1205-12 PubMed
Biochemistry. 1979 May 29;18(11):2210-8 PubMed
Folia Microbiol (Praha). 1998;43(6):623-5 PubMed
J Cell Biol. 1982 Oct;95(1):189-96 PubMed
Folia Microbiol (Praha). 1999;44(3):295-8 PubMed
Biochem Mol Biol Int. 1994 Aug;33(6):1145-9 PubMed
J Photochem Photobiol B. 1995 Jul;29(1):17-22 PubMed
Passing of fluorescein derivatives into the hyphae of Phanerochaete chrysosporium
